Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NPHP1 / Nephronophthisis Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G123301-100
Catalog Number: LS-G123301
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Nephronophthisis(NPHP1) (NM_000272), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- JBTS4 | Nephrocystin 1 | Nephronophthisis | SLSN1 | Nephrocystin-1 | Nephronophthisis 1 (juvenile) | NPH1 | NPHP1
- Origin Species
- Human
- NCBI GenBank
- NM_000272
- SwissProt
- O15259
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 83.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLARRQRDPL QALRRRNQEL KQQVDSLLSE SQLKEALEPN KRQHIYQRCI QLKQAIDENK61NALQKLSKAD ESAPVANYNQ RKEEEHTLLD KLTQQLQGLA VTISRENITE VGAPTEEEEE121SESEDSEDSG GEEEDAEEEE EEKEENESHK WSTGEEYIAV GDFTAQQVGD LTFKKGEILL181VIEKKPDGWW IAKDAKGNEG LVPRTYLEPY SEEEEGQESS EEGSEEDVEA VDETADGAEV241KQRTDPHWSA VQKAISEAGI FCLVNHVSFC YLIVLMRNRM ETVEDTNGSE TGFRAWNVQS301RGRIFLVSKP VLQQINTVDV LTTMGAIPAG FRPSTLSQLL EEGNQFRANY FLQPELMPSQ361LAFRDLMWDA TEGTIRSRPS RISLILTLWS CKMIPLPGMS IQVLSRHVRL CLFDGNKVLS421NIHTVRATWQ PKKPKTWTFS PQVTRILPCL LDGDCFIRSN SASPDLGILF ELGISYIRNS481TGERGELSCG WVFLKLFDAS GVPIPAKTYE LFLNGGTPYE KGIEVDPSIS RRAHGSVFYQ541IMTMRRQPQL LVKLRSLNRR SRNVLSLLPE TLIGNMCSIH LLIFYRQILG DVLLKDRMSL601QSTDLISHPM LATFPMLLEQ PDVMDALRSS WAGKESTLKR SEKRDKEFLK STFLLVYHDC661VLPLLHSTRL PPFRWAEEET ETARWKVITD FLKQNQENQG ALQALLSPDG VHEPFDLSEQ721TYDFLGEMRK NAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
733 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.