Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NGEF / EPHEXIN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124315-100
Catalog Number: LS-G124315
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Ephexin 1(NGEF) (NM_001114090), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF27 | Ephexin-1 | NGEF | EPHEXIN
- Origin Species
- Human
- NCBI GenBank
- AJ238899
- SwissProt
- Q8N5V2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MELLAAAFSA ACAVDHDSST SESDARDSAA GHLPGSESSS TPGNGATPEE WPALADSPTT61LTEALRMIHP IPADSWRNLI EQIGLLYQEY RDKSTLQEIE TRRQQDAEIE DNTNGSPASE121DTPEEEEEEE EEEEPASPPE RKTLPQICLL SNPHSRFNLW QDLPEIRSSG VLEILQPEEI181KLQEAMFELV TSEASYYKSL NLLVSHFMEN ERIRKILHPS EAHILFSNVL DVLAVSERFL241LELEHRMEEN IVISDVCDIV YRYAADHFSV YITYVSNQTY QERTYKQLLQ EKAAFRELIA301QLELDPKCRG LPFSSFLILP FQRITRLKLL VQNILKRVEE RSERECTALD AHKELEMVVK361ACNEGVRKMS RTEQMISIQK KMEFKIKSVP IISHSRWLLK QGELQQMSGP KTSRTLRTKK421LFHEIYLFLF NDLLVICRQI PGDKYQVFDS APRGLLRVEE LEDQGQTLAN VFILRLLENA481DDREATYMLK ASSQSEMKRW MTSLAPNRRT KFVSFTSRLL DCPQVQCVHP YVAQQPDELT541LELADILNIL DKTDDGWIFG ERLHDQERGW FPSSMTEEIL NPKIRSQNLK ECFRVHKMDD601PQRSQNKDRR KLGSRNRQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
618 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.