NGEF / EPHEXIN Antibody
Catalog Number: LS-C667157-100
100 µg / $348.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NGEF / EPHEXIN Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G86024-20
Catalog Number: LS-G86024
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Ephexin 1(NGEF) (NM_001114090) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF27 | Ephexin-1 | NGEF | EPHEXIN
- Origin Species
- Human
- NCBI GenBank
- AJ238899
- SwissProt
- Q8N5V2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MELLAAAFSA ACAVDHDSST SESDARDSAA GHLPGSESSS TPGNGATPEE WPALADSPTT61LTEALRMIHP IPADSWRNLI EQIGLLYQEY RDKSTLQEIE TRRQQDAEIE DNTNGSPASE121DTPEEEEEEE EEEEPASPPE RKTLPQICLL SNPHSRFNLW QDLPEIRSSG VLEILQPEEI181KLQEAMFELV TSEASYYKSL NLLVSHFMEN ERIRKILHPS EAHILFSNVL DVLAVSERFL241LELEHRMEEN IVISDVCDIV YRYAADHFSV YITYVSNQTY QERTYKQLLQ EKAAFRELIA301QLELDPKCRG LPFSSFLILP FQRITRLKLL VQNILKRVEE RSERECTALD AHKELEMVVK361ACNEGVRKMS RTEQMISIQK KMEFKIKSVP IISHSRWLLK QGELQQMSGP KTSRTLRTKK421LFHEIYLFLF NDLLVICRQI PGDKYQVFDS APRGLLRVEE LEDQGQTLAN VFILRLLENA481DDREATYMLK ASSQSEMKRW MTSLAPNRRT KFVSFTSRLL DCPQVQCVHP YVAQQPDELT541LELADILNIL DKTDDGWIFG ERLHDQERGW FPSSMTEEIL NPKIRSQNLK ECFRVHKMDD601PQRSQNKDRR KLGSRNRQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
618 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
