NGEF / EPHEXIN Antibody
Catalog Number: LS-C667157-100
100 µg / $348.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NGEF / EPHEXIN Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G86025-20
Catalog Number: LS-G86025
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Ephexin 1(NGEF) (NM_019850) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF27 | Ephexin-1 | NGEF | EPHEXIN
- Origin Species
- Human
- NCBI GenBank
- AJ238899
- SwissProt
- Q8N5V2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 82.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1METRESEDLE KTRRKSASDQ WNTDNEPAKV KPELLPEKEE TSQADQDIQD KEPHCHIPIK61RNSIFNRSIR RKSKAKARDN PERNASCLAD SQDNGKSVNE PLTLNIPWSR TPPCRTAMQT121DPGAQEMSES SSTPGNGATP EEWPALADSP TTLTEALRMI HPIPADSWRN LIEQIGLLYQ181EYRDKSTLQE IETRRQQDAE IEDNTNGSPA SEDTPEEEEE EEEEEEPASP PERKTLPQIC241LLSNPHSRFN LWQDLPEIRS SGVLEILQPE EIKLQEAMFE LVTSEASYYK SLNLLVSHFM301ENERIRKILH PSEAHILFSN VLDVLAVSER FLLELEHRME ENIVISDVCD IVYRYAADHF361SVYITYVSNQ TYQERTYKQL LQEKAAFREL IAQLELDPKC RGLPFSSFLI LPFQRITRLK421LLVQNILKRV EERSERECTA LDAHKELEMV VKACNEGVRK MSRTEQMISI QKKMEFKIKS481VPIISHSRWL LKQGELQQMS GPKTSRTLRT KKLFHEIYLF LFNDLLVICR QIPGDKYQVF541DSAPRGLLRV EELEDQGQTL ANVFILRLLE NADDREATYM LKASSQSEMK RWMTSLAPNR601RTKFVSFTSR LLDCPQVQCV HPYVAQQPDE LTLELADILN ILDKTDDGWI FGERLHDQER661GWFPSSMTEE ILNPKIRSQN LKECFRVHKM DDPQRSQNKD RRKLGSRNRQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
710 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
