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NEDD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NEDD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NEDD4 antibodies →
$725.00each
Catalog Number: LS-G115496-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NEDD4 (NM_006154), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0093 | Receptor-potentiating factor 1 | NEDD-4 | NEDD4-1 | NEDD4
Origin Species
Human
NCBI GenBank
D42055
SwissProt
P46934

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
104 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATCAVEVFG LLEDEENSRI VRVRVIAGIG LAKKDILGAS DPYVRVTLYD PMNGVLTSVQ
61TKTIKKSLNP KWNEEILFRV HPQQHRLLFE VFDENRLTRD DFLGQVDVPL YPLPTENPRL
121ERPYTFKDFV LHPRSHKSRV KGYLRLKMTY LPKTSGSEDD NAEQAEELEP GWVVLDQPDA
181ACHLQQQQEP SPLPPGWEER QDILGRTYYV NHESRRTQWK RPTPQDNLTD AENGNIQLQA
241QRAFTTRRQI SEETESVDNQ ESSENWEIIR EDEATMYSSQ AFPSPPPSSN LDVPTHLAEE
301LNARLTIFGN SAVSQPASSS NHSSRRGSLQ AYTFEEQPTL PVLLPTSSGL PPGWEEKQDE
361RGRSYYVDHN SRTTTWTKPT VQATVETSQL TSSQSSAGPQ SQASTSDSGQ QVTQPSEIEQ
421GFLPKGWEVR HAPNGRPFFI DHNTKTTTWE DPRLKIPAHL RGKTSLDTSN DLGPLPPGWE
481ERTHTDGRIF YINHNIKRTQ WEDPRLENVA ITGPAVPYSR DYKRKYEFFR RKLKKQNDIP
541NKFEMKLRRA TVLEDSYRRI MGVKRADFLK ARLWIEFDGE KGLDYGGVAR EWFFLISKEM
601FNPYYGLFEY SATDNYTLQI NPNSGLCNED HLSYFKFIGR VAGMAVYHGK LLDGFFIRPF
661YKMMLHKPIT LHDMESVDSE YYNSLRWILE NDPTELDLRF IIDEELFGQT HQHELKNGGS
721EIVVTNKNKK EYIYLVIQWR FVNRIQKQMA AFKEGFFELI PQDLIKIFDE NELELLMCGL
781GDVDVNDWRE HTKYKNGYSA NHQVIQWFWK AVLMMDSEKR IRLLQFVTGT SRVPMNGFAE
841LYGSNGPQSF TVEQWGTPEK LPRAHTCFNR LDLPPYESFE ELWDKLQMAI ENTQGFDGVD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

900 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.