Anti-Nedd4 Antibody - Western Blot. Western blot of affinity purified anti-Nedd4 antibody shows detection of a 115 kD band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. The blot was blocked with B501-0500 5% BLOTTO overnight at 4C. Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20000 dilution of HRP goat anti-rabbit IgG in #MB-070 Blocking Buffer for Fluorescent Western Blot. ECL was used for detection.

Polyclonal Rabbit anti-Human NEDD4 Antibody (Internal, IF, WB)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Cynomolgus Monkey, Human
Format:	Liquid

$579.00each

Catalog Number: LS-C60145-100

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Overview

Description: NEDD4 antibody LS-C60145 is an unconjugated rabbit polyclonal antibody to NEDD4 (Internal) from human. It is reactive with human, horse and macaque. Validated for ELISA, IF and WB.
Application: ELISA, IF, IHC, WB
Reactivity: Cyno, Hu
Predicted Reactivity: Bov, Can, Cyno, Hr, NHP, Rb
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: KIAA0093 | Receptor-potentiating factor 1 | NEDD-4 | NEDD4-1 | NEDD4
Recommended Dilution: ELISA: 1:20000 - 1:35000
Recommended Dilution: WB: 1:300 - 1:500
UniProt Number: P46934
NCBI GenBank: BAA07655.1, D42055
SwissProt: P46934

Target

Target: Human NEDD4
Antigen Species: Hu
Epitope: This antibody reacts with endogenous Nedd4 protein. A BLAST analysis was used to suggest reactivity with Nedd4 from human, horse and macaque based on a 100% homology with the immunizing sequence. Expect reactivity with Nedd4 from chimpanzee, rabbit, dog, and cattle based on a 94% homology with the immunizing sequence. Cross-reactivity with Nedd4 from other sources has not been determined.
Gene Name: NEDD4

Antibody

Host Species: Rb
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of the Nedd4 protein.
Immunogen Type: Pep

Format

Conjugate/Label: Unconj
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 0.64 mg/mL
Preservative: Yes
Purification Method: AP