NEDD4 Antibody
Catalog Number: LS-C667113-100
100 µg / $338.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NEDD4 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G77611-20
Catalog Number: LS-G77611
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NEDD4 (NM_198400) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0093 | Receptor-potentiating factor 1 | NEDD-4 | NEDD4-1 | NEDD4
- Origin Species
- Human
- NCBI GenBank
- D42055
- SwissProt
- P46934
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 140.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQSLRLHFA ARRSNTYPLS ETSGDDLDSH VHMCFKRPTR ISTSNVVQMK LTPRQTALAP61LIKENVQSQE RSSVPSSENV NKKSSCLQIS LQPTRYSGYL QSSNVLADSD DASFTCILKD121GIYSSAVVDN ELNAVNDGHL VSSPAICSGS LSNFSTSDNG SYSSNGSDFG SCASITSGGS181YTNSVISDSS SYTFPPSDDT FLGGNLPSDS TSNRSVPNRN TTPCEIFSRS TSTDPFVQDD241LEHGLEIMKL PVSRNTKIPL KRYSSLVIFP RSPSTTRPTS PTSLCTLLSK GSYQTSHQFI301ISPSEIAHNE DGTSAKGFLS TAVNGLRLSK TICTPGEVRD IRPLHRKGSL QKKIVLSNNT361PRQTVCEKSS EGYSCVSVHF TQRKAATLDC ETTNGDCKPE MSEIKLNSDS EYIKLMHRTS421ACLPSSQNVD CQININGELE RPHSQMNKNH GILRRSISLG GAYPNISCLS SLKHNCSKGG481PSQLLIKFAS GNEGKVDNLS RDSNRDCTNE LSNSCKPGWV VLDQPDAACH LQQQQEPSPL541PPGWEERQDI LGRTYYVNHE SRRTQWKRPT PQDNLTDAEN GNIQLQAQRA FTTRRQISEE601TESVDNRESS ENWEIIREDE ATMYSNQAFP SPPPSSNLDV PTHLAEELNA RLTIFGNSAV661SQPASSSNHS SRRGSLQAYT FEEQPTLPVL LPTSSGLPPG WEEKQDERGR SYYVDHNSRT721TTWTKPTVQA TVETSQLTSS QSSAGPQSQA STSDSGQQVT QPSEIEQGFL PKGWEVRHAP781NGRPFFIDHN TKTTTWEDPR LKIPAHLRGK TSLDTSNDLG PLPPGWEERT HTDGRIFYIN841HNIKRTQWED PRLENVAITG PAVPYSRDYK RKYEFFRRKL KKQNDIPNKF EMKLRRATVL901EDSYRRIMGV KRADFLKARL WIEFDGEKGL DYGGVAREWF FLISKEMFNP YYGLFEYSAT961DNYTLQINPN SGLCNEDHLS YFKFIGRVAG MAVYHGKLLD GFFIRPFYKM MLHKPITLHD1021MESVDSEYYN SLRWILENDP TELDLRFIID EELFGQTHQH ELKNGGSEIV VTNKNKKEYI1081YLVIQWRFVN RIQKQMAAFK EGFFELIPQD LIKIFDENEL ELLMCGLGDV DVNDWREHTK1141YKNGYSANHQ VIQWFWKAVL MMDSEKRIRL LQFVTGTSRV PMNGFAELYG SNGPQSFTVE1201QWGTPEKLPR AHTCFNRLDL PPYESFEELW DKLQMAIENT QGFDGVDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1247 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
