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MIER2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MIER2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MIER2 antibodies →
$504.00each
Catalog Number: LS-G112370-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MIER2 (NM_017550), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1193 | MIER2 | Mi-er2
Origin Species
Human
SwissProt
Q8N344

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEASSLGRQ SPRVVSCLEH SLCPGEPGLQ TTAVVSMGSG DHQFNLAEIL SQNYSVRGEC
61EEASRCPDKP KEELEKDFIS QSNDMPFDEL LALYGYEASD PISDRESEGG DVAPNLPDMT
121LDKEQIAKDL LSGEEEEETQ SSADDLTPSV TSHEASDLFP NRSGSRFLAD EDREPGSSAS
181SDTEEDSLPA NKCKKEIMVG PQFQADLSNL HLNRHCEKIY ENEDQLLWDP SVLPEREVEE
241FLYRAVKRRW HEMAGPQLPE GEAVKDSEQA LYELVKCNFN VEEALRRLRF NVKVIRDGLC
301AWSEEECRNF EHGFRVHGKN FHLIQANKVR TRSVGECVEY YYLWKKSERY DYFAQQTRLG
361RRKYVPSGTT DADQDLDGSD PDGPGRPRPE QDTLTGMRTD PLSVDGTAGG LDEPGVASDG
421LPSSEPGPCS FQQLDESPAV PLSHRPPALA DPASYQPAVT APEPDASPRL AVDFALPKEL
481PLISSHVDLS GDPEETVAPA QVALSVTEFG LIGIGDVNPF LAAHPTCPAP GLHSEPLSHC
541NVMTC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

545 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.