MIER2 Antibody
Catalog Number: LS-C754433-100
100 µg / $348.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MIER2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93834-20
Catalog Number: LS-G93834
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MIER2 (NM_017550) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1193 | MIER2 | Mi-er2
- Origin Species
- Human
- SwissProt
- Q8N344
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEASSLGRQ SPRVVSCLEH SLCPGEPGLQ TTAVVSMGSG DHQFNLAEIL SQNYSVRGEC61EEASRCPDKP KEELEKDFIS QSNDMPFDEL LALYGYEASD PISDRESEGG DVAPNLPDMT121LDKEQIAKDL LSGEEEEETQ SSADDLTPSV TSHEASDLFP NRSGSRFLAD EDREPGSSAS181SDTEEDSLPA NKCKKEIMVG PQFQADLSNL HLNRHCEKIY ENEDQLLWDP SVLPEREVEE241FLYRAVKRRW HEMAGPQLPE GEAVKDSEQA LYELVKCNFN VEEALRRLRF NVKVIRDGLC301AWSEEECRNF EHGFRVHGKN FHLIQANKVR TRSVGECVEY YYLWKKSERY DYFAQQTRLG361RRKYVPSGTT DADQDLDGSD PDGPGRPRPE QDTLTGMRTD PLSVDGTAGG LDEPGVASDG421LPSSEPGPCS FQQLDESPAV PLSHRPPALA DPASYQPAVT APEPDASPRL AVDFALPKEL481PLISSHVDLS GDPEETVAPA QVALSVTEFG LIGIGDVNPF LAAHPTCPAP GLHSEPLSHC541NVMTCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
545 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
