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MARK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MARK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112444-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MARK2(MARK2) (NM_017490), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ELKL motif kinase 1 | EMK-1 | ELKL motif kinase | EMK1 | MARK2 | PAR-1 | PAR1 homolog | Par1b | Par-1b | Ser/Thr protein kinase PAR-1B | Emk | PAR1 homolog b
Origin Species
Human
NCBI GenBank
NM_017490
SwissProt
Q7KZI7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIRGRNSATS ADEQPHIGNY RLLKTIGKGN FAKVKLARHI LTGKEVAVKI IDKTQLNSSS
61LQKLFREVRI MKVLNHPNIV KLFEVIETEK TLYLVMEYAS GGEVFDYLVA HGRMKEKEAR
121AKFRQIVSAV QYCHQKFIVH RDLKAENLLL DADMNIKIAD FGFSNEFTFG NKLDTFCGSP
181PYAAPELFQG KKYDGPEVDV WSLGVILYTL VSGSLPFDGQ NLKELRERVL RGKYRIPFYM
241STDCENLLKK FLILNPSKRG TLEQIMKDRW MNVGHEDDEL KPYVEPLPDY KDPRRTELMV
301SMGYTREEIQ DSLVGQRYNE VMATYLLLGY KSSELEGDTI TLKPRPSADL TNSSAPSPSH
361KVQRSVSANP KQRRFSDQAG PAIPTSNSYS KKTQSNNAEN KRPEEDRESG RKASSTAKVP
421ASPLPGLERK KTTPTPSTNS VLSTSTNRSR NSPLLERASL GQASIQNGKD SLTMPGSRAS
481TASASAAVSA ARPRQHQKSM SASVHPNKAS GLPPTESNCE VPRPSTAPQR VPVASPSAHN
541ISSSGGAPDR TNFPRGVSSR STFHAGQLRQ VRDQQNLPYG VTPASPSGHS QGRRGASGSI
601FSKFTSKFVR RNLNEPESKD RVETLRPHVV GSGGNDKEKE EFREAKPRSL RFTWSMKTTS
661SMEPNEMMRE IRKVLDANSC QSELHEKYML LCMHGTPGHE DFVQWEMEVC KLPRLSLNGV
721RFKRISGTSM AFKNIASKIA NELKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

745 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.