MARK2 Antibody
Catalog Number: LS-C804557
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MARK2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G78734-20
Catalog Number: LS-G78734
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MARK2(MARK2) (NM_004954) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ELKL motif kinase 1 | EMK-1 | ELKL motif kinase | EMK1 | MARK2 | PAR-1 | PAR1 homolog | Par1b | Par-1b | Ser/Thr protein kinase PAR-1B | Emk | PAR1 homolog b
- Origin Species
- Human
- NCBI GenBank
- NM_017490
- SwissProt
- Q7KZI7
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSARTPLPT LNERDTEQPT LGHLDSKPSS KSNMIRGRNS ATSADEQPHI GNYRLLKTIG61KGNFAKVKLA RHILTGKEVA VKIIDKTQLN SSSLQKLFRE VRIMKVLNHP NIVKLFEVIE121TEKTLYLVME YASGGEVFDY LVAHGRMKEK EARAKFRQIV SAVQYCHQKF IVHRDLKAEN181LLLDADMNIK IADFGFSNEF TFGNKLDTFC GSPPYAAPEL FQGKKYDGPE VDVWSLGVIL241YTLVSGSLPF DGQNLKELRE RVLRGKYRIP FYMSTDCENL LKKFLILNPS KRGTLEQIMK301DRWMNVGHED DELKPYVEPL PDYKDPRRTE LMVSMGYTRE EIQDSLVGQR YNEVMATYLL361LGYKSSELEG DTITLKPRPS ADLTNSSAPS PSHKVQRSVS ANPKQRRFSD QAGPAIPTSN421SYSKKTQSNN AENKRPEEDR ESGRKASSTA KVPASPLPGL ERKKTTPTPS TNSVLSTSTN481RSRNSPLLER ASLGQASIQN GKDSTAPQRV PVASPSAHNI SSSGGAPDRT NFPRGVSSRS541TFHAGQLRQV RDQQNLPYGV TPASPSGHSQ GRRGASGSIF SKFTSKFVRR NLNEPESKDR601VETLRPHVVG SGGNDKEKEE FREAKPRSLR FTWSMKTTSS MEPNEMMREI RKVLDANSCQ661SELHEKYMLL CMHGTPGHED FVQWEMEVCK LPRLSLNGVR FKRISGTSMA FKNIASKIAN721ELKLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
724 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
