Western Blot of Rabbit anti-MARK2pT595 antibody. Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 µg per lane. Primary antibody: MARK2 pT595 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800® rabbit secondary antibody at 1:10,000 for 45 min at RT.

MARK2 Antibody (phospho-Thr595)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$579.00each

Catalog Number: LS-C18889-100

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Quantity

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Overview

Description: MARK2 antibody LS-C18889 is an unconjugated rabbit polyclonal antibody to MARK2 (pThr595) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Application: ELISA, IF, IHC, IHC-P, WB
Reactivity: Hu
Predicted Reactivity: Bovine, Dog, Mouse, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: ELKL motif kinase 1 | EMK-1 | ELKL motif kinase | EMK1 | MARK2 | PAR-1 | PAR1 homolog | Par1b | Par-1b | Ser/Thr protein kinase PAR-1B | Emk | PAR1 homolog b
Recommended Dilution: ELISA: 1:1000 - 1:3000
Recommended Dilution: WB: 1:250 - 1:1000
UniProt Number: Q7KZI7
NCBI GenBank: NM_017490 | NP_059672.2
SwissProt: Q7KZI7

Target

Target: Human MARK2
Antigen Species: Human
Epitope: Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. A BLAST analysis was used to suggest reactivity with this protein from human, dog, bovine, mouse and rat sources based on 100% homology for the immunogen sequence. Cross reactivity with MARK2 homologues from other sources has not been determined.
Gene Name: MARK2

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store at 4°C or -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl