Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAPKAP1 / MIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122019-100
Catalog Number: LS-G122019
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIN1(MAPKAP1) (NM_001006621), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MAPKAP1 | MSIN1 | SAPK-interacting protein 1 | SIN1b | TORC2 subunit MAPKAP1 | Ras inhibitor MGC2745 | SIN1 | SIN1g | JC310 | MEKK2-interacting protein 1 | Mip1
- Origin Species
- Human
- NCBI GenBank
- NM_001006617
- SwissProt
- Q9BPZ7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTVVTMASAR VQDLIGLICW QYTSEGREPK LNDNVSAYCL HIAEDDGEVD TDFPPLDSNE61PIHKFGFSTL ALVEKYSSPG LTSKESLFVR INAAHGFSLI QVDNTKVTMK EILLKAVKRR121KGSQKVSGPQ YRLEKQSEPN VAVDLDSTLE SQSAWEFCLV RENSSRADGV FEEDSQIDIA181TVQDMLSSHH YKSFKVSMIH RLRFTTDVQL GISGDKVEID PVTNQKASTK FWIKQKPISI241DSDLLCACDL AEEKSPSHAI FKLTYLSNHD YKHLYFESDA ATVNEIVLKV NYILESRAST301ARADYFAQKQ RKLNRRTSFS FQKEKKSGQQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
330 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.