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Human MAPKAP1 / MIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MAPKAP1 / MIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G81294-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SIN1(MAPKAP1) (NM_001006621) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MAPKAP1 | MSIN1 | SAPK-interacting protein 1 | SIN1b | TORC2 subunit MAPKAP1 | Ras inhibitor MGC2745 | SIN1 | SIN1g | JC310 | MEKK2-interacting protein 1 | Mip1
Origin Species
Human
NCBI GenBank
NM_001006617
SwissProt
Q9BPZ7

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
37.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MTVVTMASAR VQDLIGLICW QYTSEGREPK LNDNVSAYCL HIAEDDGEVD TDFPPLDSNE
61PIHKFGFSTL ALVEKYSSPG LTSKESLFVR INAAHGFSLI QVDNTKVTMK EILLKAVKRR
121KGSQKVSGPQ YRLEKQSEPN VAVDLDSTLE SQSAWEFCLV RENSSRADGV FEEDSQIDIA
181TVQDMLSSHH YKSFKVSMIH RLRFTTDVQL GISGDKVEID PVTNQKASTK FWIKQKPISI
241DSDLLCACDL AEEKSPSHAI FKLTYLSNHD YKHLYFESDA ATVNEIVLKV NYILESRAST
301ARADYFAQKQ RKLNRRTSFS FQKEKKSGQQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

330 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.