MAPKAP1 / MIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110039-100
Catalog Number: LS-G110039
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIN1(MAPKAP1) (NM_024117), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MAPKAP1 | MSIN1 | SAPK-interacting protein 1 | SIN1b | TORC2 subunit MAPKAP1 | Ras inhibitor MGC2745 | SIN1 | SIN1g | JC310 | MEKK2-interacting protein 1 | Mip1
- Origin Species
- Human
- NCBI GenBank
- NM_001006617
- SwissProt
- Q9BPZ7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAFLDNPTII LAHIRQSHVT SDDTGMCEMV LIDHDVDLEK IHPPSMPGDS GSEIQGSNGE61TQGYVYAQSV DITSSWDFGI RRRSNTAQRL ERLRKERQNQ IKCKNIQWKE RNSKQSAQEL121KSLFEKKSLK EKPPISGKQS ILSVRLEQCP LQLNNPFNEY SKFDGKGHVG TTATKKIDVY181LPLHSSQDRL LPMTVVTMAS ARVQDLIGLI CWQYTSEGRE PKLNDNVSAY CLHIAEDDGE241VDTDFPPLDS NEPIHKFGFS TLALVEKYSS PGLTSKESLF VRINAAHGFS LIQVDNTKVT301MKEILLKAVK RRKGSQKVSG SRADGVFEED SQIDIATVQD MLSSHHYKSF KVSMIHRLRF361TTDVQLGISG DKVEIDPVTN QKASTKFWIK QKPISIDSDL LCACDLAEEK SPSHAIFKLT421YLSNHDYKHL YFESDAATVN EIVLKVNYIL ESRASTARAD YFAQKQRKLN RRTSFSFQKE481KKSGQQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
486 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.