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MAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MAP2 antibodies →
$725.00each
Catalog Number: LS-G120617-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP2 (NM_001039538), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MAP2C | MAP-2 | MAP2 | MAP2A | MAP2B
Origin Species
Human
NCBI GenBank
NM_002374
SwissProt
P11137

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADERKDEAK APHWTSAPLT EASAHSHPPE IKDQGGAGEG LVRSANGFPY REDEEGAFGE
61HGSQGTYSNT KENGINGELT SADRETAEEV SARIVQVVTA EAVAVLKGEQ EKEAQHKDQT
121AALPLAAEET ANLPPSPPPS PASEQTVTVE EAAGGESALA PSVFKQAKDK VSNSTLSKIP
181ALQGSTKSPR YSSACPSTTK RATFSDSLLI QPTSAGSTDR LPYSKSGNKD GVTKSPEKRS
241SLPRPSSILP PRRGVSGDRD ENSFSLNSSI SSSARRTTRS EPIRRAGKSG TSTPTTPGST
301AITPGTPPSY SSRTPGTPGT PSYPRTPHTP GTPKSAILVP SEKKVAIIRT PPKSPATPKQ
361LRLINQPLPD LKNVKSKIGS TDNIKYQPKG GQVRILNKKI DFSKVQSRCG SKDNIKHSAG
421GGNVQIVTKK IDLSHVTSKC GSLKNIRHRP GGGRVKIESV KLDFKEKAQA KVGSLDNAHH
481VPGGGNVKID SQKLNFREHA KARVDHGAEI ITQSPGRSSV ASPRRLSNVS SSGSINLLES
541PQLATLAEDV TAALAKQGL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

559 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.