MAP2 Antibody
Catalog Number: LS-C204536-50
50 µL / $426.00
Antibody: Chicken Polyclonal
Applications: Immunocytochemistry, Immunofluorescence, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G87703-20
Catalog Number: LS-G87703
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MAP2 (NM_031847) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MAP2C | MAP-2 | MAP2 | MAP2A | MAP2B
- Origin Species
- Human
- NCBI GenBank
- NM_002374
- SwissProt
- P11137
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADERKDEAK APHWTSAPLT EASAHSHPPE IKDQGGAGEG LVRSANGFPY REDEEGAFGE61HGSQGTYSNT KENGINGELT SADRETAEEV SARIVQVVTA EAVAVLKGEQ EKEAQHKDQT121AALPLAAEET ANLPPSPPPS PASEQTVTVE EAAGGESALA PSVFKQAKDK VSDGVTKSPE181KRSSLPRPSS ILPPRRGVSG DRDENSFSLN SSISSSARRT TRSEPIRRAG KSGTSTPTTP241GSTAITPGTP PSYSSRTPGT PGTPSYPRTP HTPGTPKSAI LVPSEKKVAI IRTPPKSPAT301PKQLRLINQP LPDLKNVKSK IGSTDNIKYQ PKGGQVRILN KKIDFSKVQS RCGSKDNIKH361SAGGGNVQIV TKKIDLSHVT SKCGSLKNIR HRPGGGRVKI ESVKLDFKEK AQAKVGSLDN421AHHVPGGGNV KIDSQKLNFR EHAKARVDHG AEIITQSPGR SSVASPRRLS NVSSSGSINL481LESPQLATLA EDVTAALAKQ GLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
502 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
