Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LIMS1 / PINCH Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130905-100
Catalog Number: LS-G130905
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LIMS1 (NM_001193484), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PINCH | PINCH-1 | PINCH1 | LIMS1
- Origin Species
- Human
- NCBI GenBank
- NM_004987
- SwissProt
- P48059
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTALQLKELS HSGLYRRRRD RPDSLRVNGL PEEELSNMAN ALASATCERC KGGFAPAEKI61VNSNGELYHE QCFVCAQCFQ QFPEGLFYEF EGRKYCEHDF QMLFAPCCHQ CGEFIIGRVI121KAMNNSWHPE CFRCDLCQEV LADIGFVKNA GRHLCRPCHN REKARGLGKY ICQKCHAIID181EQPLIFKNDP YHPDHFNCAN CGKELTADAR ELKGELYCLP CHDKMGVPIC GACRRPIEGR241VVNAMGKQWH VEHFVCAKCE KPFLGHRHYE RKGLAYCETH YNQLFGDVCF HCNRVIEGDV301VSALNKAWCV NCFACSTCNT KLTLKNKFVE FDMKPVCKKC YEKFPLELKK RLKKLAETLG361RKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
362 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.