Immunohistochemistry image at a dilution of 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-plus™ LIMS1 / PINCH Antibody

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Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human, Rat
Format:	Liquid

$383.00each

Catalog Number: LS-B16025-50

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Overview

Description: PINCH antibody LS-B16025 is an unconjugated rabbit polyclonal antibody to PINCH (LIMS1) from human. It is reactive with human and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: ELISA, IHC, IHC-P, WB
Reactivity: Hu, Rt
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: PINCH | PINCH-1 | PINCH1 | LIMS1
Recommended Dilution: IHC-P: 10 µg/ml
Recommended Dilution: WB: 1:20 - 1:200
UniProt Number: P48059
NCBI GenBank: NM_004987 | NP_004978.2
SwissProt: P48059