Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ITCH / AIP4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102004-100
Catalog Number: LS-G102004
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITCH(ITCH) (NM_031483), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AIF4 | AIP4 | DJ468O1.1 | ITCH | NAPP1 | NFE2-associated polypeptide 1
- Origin Species
- Human
- NCBI GenBank
- NM_031483
- SwissProt
- Q96J02
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDSGSQLGS MGSLTMKSQL QITVISAKLK ENKKNWFGPS PYVEVTVDGQ SKKTEKCNNT61NSPKWKQPLT VIVTPVSKLH FRVWSHQTLK SDVLLGTAAL DIYETLKSNN MKLEEVVVTL121QLGGDKEPTE TIGDLSICLD GLQLESEVVT NGETTCSESA SQNDDGSRSK DETRVSTNGS181DDPEDAGAGE NRRVSGNNSP SLSNGGFKPS RPPRPSRPPP PTPRRPASVN GSPSATSESD241GSSTGSLPPT NTNTNTSEGA TSGLIIPLTI SGGSGPRPLN PVTQAPLPPG WEQRVDQHGR301VYYVDHVEKR TTWDRPEPLP PGWERRVDNM GRIYYVDHFT RTTTWQRPTL ESVRNYEQWQ361LQRSQLQGAM QQFNQRFIYG NQDLFATSQS KEFDPLGPLP PGWEKRTDSN GRVYFVNHNT421RITQWEDPRS QGQLNEKPLP EGWEMRFTVD GIPYFVDHNR RTTTYIDPRT GKSALDNGPQ481IAYVRDFKAK VQYFRFWCQQ LAMPQHIKIT VTRKTLFEDS FQQIMSFSPQ DLRRRLWVIF541PGEEGLDYGG VAREWFFLLS HEVLNPMYCL FEYAGKDNYC LQINPASYIN PDHLKYFRFI601GRFIAMALFH GKFIDTGFSL PFYKRILNKP VGLKDLESID PEFYNSLIWV KENNIEECDL661EMYFSVDKEI LGEIKSHDLK PNGGNILVTE ENKEEYIRMV AEWRLSRGVE EQTQAFFEGF721NEILPQQYLQ YFDAKELEVL LCGMQEIDLN DWQRHAIYRH YARTSKQIMW FWQFVKEIDN781EKRMRLLQFV TGTCRLPVGG FADLMGSNGP QKLCIEKVGK ENWLPRSHTC FNRLDLPPYK841SYEQLKEKLL FAIEETEGFG QETRTRPLEQKLISEEDLAANDILDYKDDDDKV
862 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.