Detection of Human Itch by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Itch antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
ITCH / AIP4 Antibody (aa650-700)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$503.00each
Catalog Number: LS-C288763-100
Catalog Number: LS-C288763
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- Description
- AIP4 antibody LS-C288763 is an unconjugated rabbit polyclonal antibody to human AIP4 (ITCH) (aa650-700). Validated for WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AIF4 | AIP4 | DJ468O1.1 | ITCH | NAPP1 | NFE2-associated polypeptide 1
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- Q96J02
- NCBI GenBank
- NM_031483 | NP_113671.3
- SwissProt
- Q96J02
Target
- Target
- Human ITCH / AIP4
- Antigen Species
- Human
- Epitope
- Region between residue 650 to 700 of human itchy homolog E3 ubiquitin protein ligase using the numbering given in entry AAH11571.1 (GeneID 83737).
- Gene Name
- ITCH
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 83737 corresponding to amino acids between 650 and 700 of UniprotID: Q96J02
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- TBS, 0.09% Sodium Azide, 0.1% BSA
- Concentration
- 0.2 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- TBS