LSBio
Polyclonal Rabbit anti-Human ITCH / AIP4 Antibody (Preservative Free, IF)

Immunofluorescence staining of ITCH in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ITCH polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.

Polyclonal Rabbit anti-Human ITCH / AIP4 Antibody (Preservative Free, IF)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence
Reactivity:Human
Format:Liquid
See all Human ITCH / AIP4 antibodies →
$309.00each
Catalog Number: LS-C818113-100

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Overview

Description
AIP4 antibody LS-C818113 is an unconjugated rabbit polyclonal antibody to human AIP4 (ITCH). Validated for ELISA, ICC and IF.
Application
ELISA, ICC, IF
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AIF4 | AIP4 | DJ468O1.1 | ITCH | NAPP1 | NFE2-associated polypeptide 1
Recommended Dilution: ELISA
1:5000 - 1:10000
Recommended Dilution: IF/ICC
1:100 - 1:500
UniProt Number
Q96J02
SwissProt
Q96J02

Target

Target
Human ITCH / AIP4
Antigen Species
Human
Epitope
Human ITCH
Gene Name
ITCH

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant Human ITCH Protein
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.2 µm filtered solution in PBS
Volume
100 Microliter
Preservative
No
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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