Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IBSP / Bone Sialoprotein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116281-100
Catalog Number: LS-G116281
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Bone Sialoprotein(IBSP) (NM_004967), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Bone Sialoprotein | Bone sialoprotein II | BSP | BSP II | Cell-binding sialoprotein | BNSP | Bone Sialprotein II | IBSP | Integrin-binding sialoprotein | Bone sialoprotein 2 | BSP-II | SP-II
- Origin Species
- Human
- NCBI GenBank
- NM_004967
- SwissProt
- P21815
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.02 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKTALILLSI LGMACAFSMK NLHRRVKIED SEENGVFKYR PRYYLYKHAY FYPHLKRFPV61QGSSDSSEEN GDDSSEEEEE EEETSNEGEN NEESNEDEDS EAENTTLSAT TLGYGEDATP121GTGYTGLAAI QLPKKAGDIT NKATKEKESD EEEEEEEEGN ENEESEAEVD ENEQGINGTS181TNSTEAENGN GSSGGDNGEE GEEESVTGAN AEGTTETGGQ GKGTSKTTTS PNGGFEPTTP241PQVYRTTSPP FGKTTTVEYE GEYEYTGVNE YDNGYEIYES ENGEPRGDNY RAYEDEYSYF301KGQGYDGYDG QNYYHHQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
317 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.