LSBio
Human ZEB1 / AREB6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ZEB1 / AREB6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G77713-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AREB6(ZEB1) (NM_030751) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AREB6 | BZP | FECD6 | NIL-2-A zinc finger protein | TCF-8 | Negative regulator of IL2 | NIL2A | ZEB | ZEB1 | ZFHEP | DELTAEF1 | NIL-2-A | PPCD3 | TCF8 | Transcription factor 8 | ZFHX1A
Origin Species
Human
NCBI GenBank
NM_030751
SwissProt
P37275

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADGPRCKRR KQANPRRNNV TNYNTVVETN SDSDDEDKLH IVEEESVTDA ADCEGVPEDD
61LPTDQTVLPG RSSEREGNAK NCWEDDTGKE GQEILGPEAQ ADEAGCTVKD DECESDAENE
121QNHDPNVEEF LQQQDTAVIF PEAPEEDQRQ GTPEASGHDE NGTPDAFSQL LTCPYCDRGY
181KRFTSLKEHI KYRHEKNEDN FSCSLCSYTF AYRTQLERHM TSHKSGRDQR HVTQSGCNRK
241FKCTECGKAF KYKHHLKEHL RIHSGEKPYE CPNCKKRFSH SGSYSSHISS KKCISLIPVN
301GRPRTGLKTS QCSSPSLSAS PGSPTRPQIR QKIENKPLQE QLSVNQIKTE PVDYEFKPIV
361VASGINCSTP LQNGVFTGGG PLQATSSPQG MVQAVVLPTV GLVSPISINL SDIQNVLKVA
421VDGNVIRQVL ENNQANLASK EQETINASPI QQGGHSVISA ISLPLVDQDG TTKIIINYSL
481EQPSQLQVVP QNLKKENPVA TNSCKSEKLP EDLTVKSEKD KSFEGGVNDS TCLLCDDCPG
541DINALPELKH YDLKQPTQPP PLPAAEAEKP ESSVSSATGD GNLSPSQPPL KNLLSLLKAY
601YALNAQPSAE ELSKIADSVN LPLDVVKKWF EKMQAGQISV QSSEPSSPEP GKVNIPAKNN
661DQPQSANANE PQDSTVNLQS PLKMTNSPVL PVGSTTNGSR SSTPSPSPLN LSSSRNTQGY
721LYTAEGAQEE PQVEPLDLSL PKQQGELLER STITSVYQNS VYSVQEEPLN LSCAKKEPQK
781DSCVTDSEPV VNVIPPSANP INIAIPTVTA QLPTIVAIAD QNSVPCLRAL AANKQTILIP
841QVAYTYSTTV SPAVQEPPLK VIQPNGNQDE RQDTSSEGVS NVEDQNDSDS TPPKKKMRKT
901ENGMYACDLC DKIFQKSSSL LRHKYEHTGK RPHECGICKK AFKHKHHLIE HMRLHSGEKP
961YQCDKCGKRF SHSGSYSQHM NHRYSYCKRE AEERDSTEQE EAGPEILSNE HVGARASPSQ
1021GDSDERESLT REEDEDSEKE EEEEDKEMEE LQEEKECEKP QGDEEEEEEE EEVEEEEVEE
1081AENEGEEAKT EGLMKDDRAE SQASSLGQKV GESSEQVSEE KTNEA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1125 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.