ZEB1 / AREB6 Antibody
Catalog Number: LS-C836162
2 sizes / from $305.00
Antibody: Rabbit Recombinant Monoclonal
Applications: Flow Cytometry, Immunoprecipitation, Western Blot
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ZEB1 / AREB6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G77712-20
Catalog Number: LS-G77712
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AREB6(ZEB1) (NM_001128128) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AREB6 | BZP | FECD6 | NIL-2-A zinc finger protein | TCF-8 | Negative regulator of IL2 | NIL2A | ZEB | ZEB1 | ZFHEP | DELTAEF1 | NIL-2-A | PPCD3 | TCF8 | Transcription factor 8 | ZFHX1A
- Origin Species
- Human
- NCBI GenBank
- NM_030751
- SwissProt
- P37275
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 121.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKVTNYNTVV ETNSDSDDED KLHIVEEESV TDAADCEGVP EDDLPTDQTV LPGRSSEREG61NAKNCWEDDT GKEGQEILGP EAQADEAGCT VKDDECESDA ENEQNHDPNV EEFLQQQDTA121VIFPEAPEED QRQGTPEASG HDENGTPDAF SQLLTCPYCD RGYKRFTSLK EHIKYRHEKN181EDNFSCSLCS YTFAYRTQLE RHMTSHKSGR DQRHVTQSGC NRKFKCTECG KAFKYKHHLK241EHLRIHSGEK PYECPNCKKR FSHSGSYSSH ISSKKCISLI PVNGRPRTGL KTSQCSSPSL301SASPGSPTRP QIRQKIENKP LQEQLSVNQI KTEPVDYEFK PIVVASGINC STPLQNGVFT361GGGPLQATSS PQGMVQAVVL PTVGLVSPIS INLSDIQNVL KVAVDGNVIR QVLENNQANL421ASKEQETINA SPIQQGGHSV ISAISLPLVD QDGTTKIIIN YSLEQPSQLQ VVPQNLKKEN481PVATNSCKSE KLPEDLTVKS EKDKSFEGGV NDSTCLLCDD CPGDINALPE LKHYDLKQPT541QPPPLPAAEA EKPESSVSSA TGDGNLSPSQ PPLKNLLSLL KAYYALNAQP SAEELSKIAD601SVNLPLDVVK KWFEKMQAGQ ISVQSSEPSS PEPGKVNIPA KNNDQPQSAN ANEPQDSTVN661LQSPLKMTNS PVLPVGSTTN GSRSSTPSPS PLNLSSSRNT QGYLYTAEGA QEEPQVEPLD721LSLPKQQGEL LERSTITSVY QNSVYSVQEE PLNLSCAKKE PQKDSCVTDS EPVVNVIPPS781ANPINIAIPT VTAQLPTIVA IADQNSVPCL RALAANKQTI LIPQVAYTYS TTVSPAVQEP841PLKVIQPNGN QDERQDTSSE GVSNVEDQND SDSTPPKKKM RKTENGMYAC DLCDKIFQKS901SSLLRHKYEH TGKRPHECGI CKKAFKHKHH LIEHMRLHSG EKPYQCDKCG KRFSHSGSYS961QHMNHRYSYC KREAEERDST EQEEAGPEIL SNEHVGARAS PSQGDSDERE SLTREEDEDS1021EKEEEEEDKE MEELQEEKEC EKPQGDEEEE EEEEEVEEEE VEEAENEGEE AKTEGLMKDD1081RAESQASSLG QKVGESSEQV SEEKTNEATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1108 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
