LSBio
Human ZEB1 / AREB6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ZEB1 / AREB6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G77712-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AREB6(ZEB1) (NM_001128128) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AREB6 | BZP | FECD6 | NIL-2-A zinc finger protein | TCF-8 | Negative regulator of IL2 | NIL2A | ZEB | ZEB1 | ZFHEP | DELTAEF1 | NIL-2-A | PPCD3 | TCF8 | Transcription factor 8 | ZFHX1A
Origin Species
Human
NCBI GenBank
NM_030751
SwissProt
P37275

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
121.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKVTNYNTVV ETNSDSDDED KLHIVEEESV TDAADCEGVP EDDLPTDQTV LPGRSSEREG
61NAKNCWEDDT GKEGQEILGP EAQADEAGCT VKDDECESDA ENEQNHDPNV EEFLQQQDTA
121VIFPEAPEED QRQGTPEASG HDENGTPDAF SQLLTCPYCD RGYKRFTSLK EHIKYRHEKN
181EDNFSCSLCS YTFAYRTQLE RHMTSHKSGR DQRHVTQSGC NRKFKCTECG KAFKYKHHLK
241EHLRIHSGEK PYECPNCKKR FSHSGSYSSH ISSKKCISLI PVNGRPRTGL KTSQCSSPSL
301SASPGSPTRP QIRQKIENKP LQEQLSVNQI KTEPVDYEFK PIVVASGINC STPLQNGVFT
361GGGPLQATSS PQGMVQAVVL PTVGLVSPIS INLSDIQNVL KVAVDGNVIR QVLENNQANL
421ASKEQETINA SPIQQGGHSV ISAISLPLVD QDGTTKIIIN YSLEQPSQLQ VVPQNLKKEN
481PVATNSCKSE KLPEDLTVKS EKDKSFEGGV NDSTCLLCDD CPGDINALPE LKHYDLKQPT
541QPPPLPAAEA EKPESSVSSA TGDGNLSPSQ PPLKNLLSLL KAYYALNAQP SAEELSKIAD
601SVNLPLDVVK KWFEKMQAGQ ISVQSSEPSS PEPGKVNIPA KNNDQPQSAN ANEPQDSTVN
661LQSPLKMTNS PVLPVGSTTN GSRSSTPSPS PLNLSSSRNT QGYLYTAEGA QEEPQVEPLD
721LSLPKQQGEL LERSTITSVY QNSVYSVQEE PLNLSCAKKE PQKDSCVTDS EPVVNVIPPS
781ANPINIAIPT VTAQLPTIVA IADQNSVPCL RALAANKQTI LIPQVAYTYS TTVSPAVQEP
841PLKVIQPNGN QDERQDTSSE GVSNVEDQND SDSTPPKKKM RKTENGMYAC DLCDKIFQKS
901SSLLRHKYEH TGKRPHECGI CKKAFKHKHH LIEHMRLHSG EKPYQCDKCG KRFSHSGSYS
961QHMNHRYSYC KREAEERDST EQEEAGPEIL SNEHVGARAS PSQGDSDERE SLTREEDEDS
1021EKEEEEEDKE MEELQEEKEC EKPQGDEEEE EEEEEVEEEE VEEAENEGEE AKTEGLMKDD
1081RAESQASSLG QKVGESSEQV SEEKTNEA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1108 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.