Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZEB1 / AREB6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101957-100
Catalog Number: LS-G101957
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AREB6(ZEB1) (NM_030751), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AREB6 | BZP | FECD6 | NIL-2-A zinc finger protein | TCF-8 | Negative regulator of IL2 | NIL2A | ZEB | ZEB1 | ZFHEP | DELTAEF1 | NIL-2-A | PPCD3 | TCF8 | Transcription factor 8 | ZFHX1A
- Origin Species
- Human
- NCBI GenBank
- NM_030751
- SwissProt
- P37275
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 124.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADGPRCKRR KQANPRRNNV TNYNTVVETN SDSDDEDKLH IVEEESVTDA ADCEGVPEDD61LPTDQTVLPG RSSEREGNAK NCWEDDTGKE GQEILGPEAQ ADEAGCTVKD DECESDAENE121QNHDPNVEEF LQQQDTAVIF PEAPEEDQRQ GTPEASGHDE NGTPDAFSQL LTCPYCDRGY181KRFTSLKEHI KYRHEKNEDN FSCSLCSYTF AYRTQLERHM TSHKSGRDQR HVTQSGCNRK241FKCTECGKAF KYKHHLKEHL RIHSGEKPYE CPNCKKRFSH SGSYSSHISS KKCISLIPVN301GRPRTGLKTS QCSSPSLSAS PGSPTRPQIR QKIENKPLQE QLSVNQIKTE PVDYEFKPIV361VASGINCSTP LQNGVFTGGG PLQATSSPQG MVQAVVLPTV GLVSPISINL SDIQNVLKVA421VDGNVIRQVL ENNQANLASK EQETINASPI QQGGHSVISA ISLPLVDQDG TTKIIINYSL481EQPSQLQVVP QNLKKENPVA TNSCKSEKLP EDLTVKSEKD KSFEGGVNDS TCLLCDDCPG541DINALPELKH YDLKQPTQPP PLPAAEAEKP ESSVSSATGD GNLSPSQPPL KNLLSLLKAY601YALNAQPSAE ELSKIADSVN LPLDVVKKWF EKMQAGQISV QSSEPSSPEP GKVNIPAKNN661DQPQSANANE PQDSTVNLQS PLKMTNSPVL PVGSTTNGSR SSTPSPSPLN LSSSRNTQGY721LYTAEGAQEE PQVEPLDLSL PKQQGELLER STITSVYQNS VYSVQEEPLN LSCAKKEPQK781DSCVTDSEPV VNVIPPSANP INIAIPTVTA QLPTIVAIAD QNSVPCLRAL AANKQTILIP841QVAYTYSTTV SPAVQEPPLK VIQPNGNQDE RQDTSSEGVS NVEDQNDSDS TPPKKKMRKT901ENGMYACDLC DKIFQKSSSL LRHKYEHTGK RPHECGICKK AFKHKHHLIE HMRLHSGEKP961YQCDKCGKRF SHSGSYSQHM NHRYSYCKRE AEERDSTEQE EAGPEILSNE HVGARASPSQ1021GDSDERESLT REEDEDSEKE EEEEDKEMEE LQEEKECEKP QGDEEEEEEE EEVEEEEVEE1081AENEGEEAKT EGLMKDDRAE SQASSLGQKV GESSEQVSEE KTNEATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1125 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.