VPS18 Antibody (clone 4E9)
Catalog Number: LS-C139435-100
100 µg / $342.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human VPS18 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G83031-20
Catalog Number: LS-G83031
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human VPS18 (NM_020857) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HVPS18 | KIAA1475 | PEP3 | VPS18
- Origin Species
- Human
- NCBI GenBank
- AA082935
- SwissProt
- Q9P253
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 110 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASILDEYEN SLSRSAVLQP GCPSVGIPHS GYVNAQLEKE VPIFTKQRID FTPSERITSL61VVSSNQLCMS LGKDTLLRID LGKANEPNHV ELGRKDDAKV HKMFLDHTGS HLLIALSSTE121VLYVNRNGQK VRPLARWKGQ LVESVGWNKA LGTESSTGPI LVGTAQGHIF EAELSASEGG181LFGPAPDLYF RPLYVLNEEG GPAPVCSLEA ERGPDGRSFV IATTRQRLFQ FIGRAAEGAE241AQGFSGLFAA YTDHPPPFRE FPSNLGYSEL AFYTPKLRSA PRAFAWMMGD GVLYGALDCG301RPDSLLSEER VWEYPEGVGP GASPPLAIVL TQFHFLLLLA DRVEAVCTLT GQVVLRDHFL361EKFGPLKHMV KDSSTGQLWA YTERAVFRYH VQREARDVWR TYLDMNRFDL AKEYCRERPD421CLDTVLAREA DFCFRQRRYL ESARCYALTQ SYFEEIALKF LEARQEEALA EFLQRKLASL481KPAERTQATL LTTWLTELYL SRLGALQGDP EALTLYRETK ECFRTFLSSP RHKEWLFASR541ASIHELLASH GDTEHMVYFA VIMQDYERVV AYHCQHEAYE EALAVLARHR DPQLFYKFSP601ILIRHIPRQL VDAWIEMGSR LDARQLIPAL VNYSQGGEVQ QVSQAIRYME FCVNVLGETE661QAIHNYLLSL YARGRPDSLL AYLEQAGASP HRVHYDLKYA LRLCAEHGHH RACVHVYKVL721ELYEEAVDLA LQVDVDLAKQ CADLPEEDEE LRKKLWLKIA RHVVQEEEDV QTAMACLASC781PLLKIEDVLP FFPDFVTIDH FKEAICSSLK AYNHHIQELQ REMEEATASA QRIRRDLQEL841RGRYGTVEPQ DKCATCDFPL LNRPFYLFLC GHMFHADCLL QAVRPGLPAY KQARLEELQR901KLGAAPPPAK GSARAKEAEG GAATAGPSRE QLKADLDELV AAECVYCGEL MIRSIDRPFI961DPQRYEEEQL SWLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
973 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
