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VPS16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VPS16 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all VPS16 antibodies →
$725.00each
Catalog Number: LS-G107891-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VPS16 (NM_080413), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HVPS16 | VPS16
Origin Species
Human
NCBI GenBank
NM_022575
SwissProt
Q9H269

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDCYTANWNP LGDSAFYRKY ELYSMDWDLK EELRDCLVAA APYGGPIALL RNPWRKEKAA
61SVRPVLDIYS ASGMPLASLL WKSGPVVSLG WSAEEELLCV QEDGAVLVYG LHGDFRRHFS
121MGNEVLQNRV LDARIFHTEF GSGVAILTGA HRFTLSANVG DLKLRRMPEV PGLQSAPSCW
181TVLCQDRVAH ILLAVGPDLY LLDHAACSAV TPPGLAPGVS SFLQMAVSFT YRHLALFTDT
241GYIWMGTASL KEKLCEFNCN IRAPPKQMVW CSRPRSKERA VVVAWERRLM VVGDAPESIQ
301YKQLTIQVLL DRLVLRRLYP LAIQICEYLR LPEVQGVSRI LAHWACYKVQ QKDVSDEDVA
361RAINQKLGDT PGVSYSDIAA RAYGCGRTEL AIKLLEYEPR SGEQVPLLLK MKRSKLALSK
421AIESGDTDLV FTVLLHLKNE LNRGDFFMTL RNQPMALSLY RQFCKHQELE TLKDLYNQDD
481NHQELGSFHI RASYAAEERI EGRVAALQTA ADAFYKAKNE FAAKATEDQM RLLRLQRRLE
541DELGGQFLDL SLHDTVTTLI LGGHNKRAEQ LARDFRIPDK RLWWLKLTAL ADLEDWEELE
601KFSKSKKSPI GYLPFVEICM KQHNKYEAKK YASRVGPEQK VKALLLVGDV AQAADVAIEH
661RNEAELSLVL SHCTGATDGA TADKIQRARA QAQKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

695 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.