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VPS18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VPS18 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all VPS18 antibodies →
$504.00each
Catalog Number: LS-G110960-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VPS18 (NM_020857), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HVPS18 | KIAA1475 | PEP3 | VPS18
Origin Species
Human
NCBI GenBank
AA082935
SwissProt
Q9P253

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
110 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASILDEYEN SLSRSAVLQP GCPSVGIPHS GYVNAQLEKE VPIFTKQRID FTPSERITSL
61VVSSNQLCMS LGKDTLLRID LGKANEPNHV ELGRKDDAKV HKMFLDHTGS HLLIALSSTE
121VLYVNRNGQK VRPLARWKGQ LVESVGWNKA LGTESSTGPI LVGTAQGHIF EAELSASEGG
181LFGPAPDLYF RPLYVLNEEG GPAPVCSLEA ERGPDGRSFV IATTRQRLFQ FIGRAAEGAE
241AQGFSGLFAA YTDHPPPFRE FPSNLGYSEL AFYTPKLRSA PRAFAWMMGD GVLYGALDCG
301RPDSLLSEER VWEYPEGVGP GASPPLAIVL TQFHFLLLLA DRVEAVCTLT GQVVLRDHFL
361EKFGPLKHMV KDSSTGQLWA YTERAVFRYH VQREARDVWR TYLDMNRFDL AKEYCRERPD
421CLDTVLAREA DFCFRQRRYL ESARCYALTQ SYFEEIALKF LEARQEEALA EFLQRKLASL
481KPAERTQATL LTTWLTELYL SRLGALQGDP EALTLYRETK ECFRTFLSSP RHKEWLFASR
541ASIHELLASH GDTEHMVYFA VIMQDYERVV AYHCQHEAYE EALAVLARHR DPQLFYKFSP
601ILIRHIPRQL VDAWIEMGSR LDARQLIPAL VNYSQGGEVQ QVSQAIRYME FCVNVLGETE
661QAIHNYLLSL YARGRPDSLL AYLEQAGASP HRVHYDLKYA LRLCAEHGHH RACVHVYKVL
721ELYEEAVDLA LQVDVDLAKQ CADLPEEDEE LRKKLWLKIA RHVVQEEEDV QTAMACLASC
781PLLKIEDVLP FFPDFVTIDH FKEAICSSLK AYNHHIQELQ REMEEATASA QRIRRDLQEL
841RGRYGTVEPQ DKCATCDFPL LNRPFYLFLC GHMFHADCLL QAVRPGLPAY KQARLEELQR
901KLGAAPPPAK GSARAKEAEG GAATAGPSRE QLKADLDELV AAECVYCGEL MIRSIDRPFI
961DPQRYEEEQL SWL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

973 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.