Villin-Like / VILL Antibody
Catalog Number: LS-C750412
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human Villin-Like / VILL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G81080-20
Catalog Number: LS-G81080
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human VILL (NM_015873) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- VILL | Villin-like protein | Villin-like
- Origin Species
- Human
- NCBI GenBank
- NM_015873
- SwissProt
- O15195
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 95.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDISKGLPGM QGGLHIWISE NRKMVPVPEG AYGNFFEEHC YVILHVPQSP KATQGASSDL61HYWVGKQAGA EAQGAAEAFQ QRLQDELGGQ TVLHREAQGH ESDCFCSYFR PGIIYRKGGL121ASDLKHVETN LFNIQRLLHI KGRKHVSATE VELSWNSFNK GDIFLLDLGK MMIQWNGPKT181SISEKARGLA LTYSLRDRER GGGRAQIGVV DDEAKAPDLM QIMEAVLGRR VGSLRAATPS241KDINQLQKAN VRLYHVYEKG KDLVVLELAT PPLTQDLLQE EDFYILDQGG FKIYVWQGRM301SSLQERKAAF SRAVGFIQAK GYPTYTNVEV VNDGAESAAF KQLFRTWSEK RRRNQKLGGR361DKSIHVKLDV GKLHTQPKLA AQLRMVDDGS GKVEVWCIQD LHRQPVDPKR HGQLCAGNCY421LVLYTYQRLG RVQYILYLWQ GHQATADEIE ALNSNAEELD VMYGGVLVQE HVTMGSEPPH481FLAIFQGQLV IFQERAGHHG KGQSASTTRL FQVQGTDSHN TRTMEVPARA SSLNSSDIFL541LVTASVCYLW FGKGCNGDQR EMARVVVTVI SRKNEETVLE GQEPPHFWEA LGGRAPYPSN601KRLPEEVPSF QPRLFECSSH MGCLVLAEVG FFSQEDLDKY DIMLLDTWQE IFLWLGEAAS661EWKEAVAWGQ EYLKTHPAGR SPATPIVLVK QGHEPPTFIG WFFTWDPYKW TSHPSHKEVV721DGSPAAASTI SEITAEVNNL RLSRWPGNGR AGAVALQALK GSQDSSENDL VRSPKSAGSR781TSSSVSSTSA TINGGLRREQ LMHQAVEDLP EGVDPARREF YLSDSDFQDI FGKSKEEFYS841MATWRQRQEK KQLGFFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
856 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
