Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Villin-Like / VILL Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113015-100
Catalog Number: LS-G113015
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VILL (NM_015873), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- VILL | Villin-like protein | Villin-like
- Origin Species
- Human
- NCBI GenBank
- NM_015873
- SwissProt
- O15195
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 95.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDISKGLPGM QGGLHIWISE NRKMVPVPEG AYGNFFEEHC YVILHVPQSP KATQGASSDL61HYWVGKQAGA EAQGAAEAFQ QRLQDELGGQ TVLHREAQGH ESDCFCSYFR PGIIYRKGGL121ASDLKHVETN LFNIQRLLHI KGRKHVSATE VELSWNSFNK GDIFLLDLGK MMIQWNGPKT181SISEKARGLA LTYSLRDRER GGGRAQIGVV DDEAKAPDLM QIMEAVLGRR VGSLRAATPS241KDINQLQKAN VRLYHVYEKG KDLVVLELAT PPLTQDLLQE EDFYILDQGG FKIYVWQGRM301SSLQERKAAF SRAVGFIQAK GYPTYTNVEV VNDGAESAAF KQLFRTWSEK RRRNQKLGGR361DKSIHVKLDV GKLHTQPKLA AQLRMVDDGS GKVEVWCIQD LHRQPVDPKR HGQLCAGNCY421LVLYTYQRLG RVQYILYLWQ GHQATADEIE ALNSNAEELD VMYGGVLVQE HVTMGSEPPH481FLAIFQGQLV IFQERAGHHG KGQSASTTRL FQVQGTDSHN TRTMEVPARA SSLNSSDIFL541LVTASVCYLW FGKGCNGDQR EMARVVVTVI SRKNEETVLE GQEPPHFWEA LGGRAPYPSN601KRLPEEVPSF QPRLFECSSH MGCLVLAEVG FFSQEDLDKY DIMLLDTWQE IFLWLGEAAS661EWKEAVAWGQ EYLKTHPAGR SPATPIVLVK QGHEPPTFIG WFFTWDPYKW TSHPSHKEVV721DGSPAAASTI SEITAEVNNL RLSRWPGNGR AGAVALQALK GSQDSSENDL VRSPKSAGSR781TSSSVSSTSA TINGGLRREQ LMHQAVEDLP EGVDPARREF YLSDSDFQDI FGKSKEEFYS841MATWRQRQEK KQLGFFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
856 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.