LSBio
Human TP53BP2 / ASPP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TP53BP2 / ASPP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G87826-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TP53BP2(TP53BP2) (NM_001031685) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
53BP2 | p53-binding protein 53bp2 | p53BP2 | TP53BP2 | PPP1R13A | ASPP2 | BBP | BCL2-binding protein | p53-binding protein 2
Origin Species
Human
NCBI GenBank
NM_005426
SwissProt
Q13625

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPMFLTVYLS NNEQHFTEVP VTPETICRDV VDLCKEPGES DCHLAEVWCG SERPVADNER
61MFDVLQRFGS QRNEVRFFLR HERPPGRDIV SGPRSQDPSL KRNGVKVPGE YRRKENGVNS
121PRMDLTLAEL QEMASRQQQQ IEAQQQLLAT KEQRLKFLKQ QDQRQQQQVA EQEKLKRLKE
181IAENQEAKLK KVRALKGHVE QKRLSNGKLV EEIEQMNNLF QQKQRELVLA VSKVEELTRQ
241LEMLKNGRID SHHDNQSAVA ELDRLYKELQ LRNKLNQEQN AKLQQQRECL NKRNSEVAVM
301DKRVNELRDR LWKKKAALQQ KENLPVSSDG NLPQQAASAP SRVAAVGPYI QSSTMPRMPS
361RPELLVKPAL PDGSLVIQAS EGPMKIQTLP NMRSGAASQT KGSKIHPVGP DWSPSNADLF
421PSQGSASVPQ STGNALDQVD DGEVPLREKE KKVRPFSMFD AVDQSNAPPS FGTLRKNQSS
481EDILRDAQVA NKNVAKVPPP VPTKPKQINL PYFGQTNQPP SDIKPDGSSQ QLSTVVPSMG
541TKPKPAGQQP RVLLSPSIPS VGQDQTLSPG SKQESPPAAA VRPFTPQPSK DTLLPPFRKP
601QTVAASSIYS MYTQQQAPGK NFQQAVQSAL TKTHTRGPHF SSVYGKPVIA AAQNQQQHPE
661NIYSNSQGKP GSPEPETEPV SSVQENHENE RIPRPLSPTK LLPFLSNPYR NQSDADLEAL
721RKKLSNAPRP LKKRSSITEP EGPNGPNIQK LLYQRTTIAA METISVPSYP SKSASVTASS
781ESPVEIQNPY LHVEPEKEVV SLVPESLSPE DVGNASTENS DMPAPSPGLD YEPEGVPDNS
841PNLQNNPEEP NPEAPHVLDV YLEEYPPYPP PPYPSGEPEG PGEDSVSMRP PEITGQVSLP
901PGKRTNLRKT GSERIAHGMR VKFNPLALLL DSSLEGEFDL VQRIIYEVDD PSLPNDEGIT
961ALHNAVCAGH TEIVKFLVQF GVNVNAADSD GWTPLHCAAS CNNVQVCKFL VESGAAVFAM
1021TYSDMQTAAD KCEEMEEGYT QCSQFLYGVQ EKMGIMNKGV IYALWDYEPQ NDDELPMKEG
1081DCMTIIHRED EDEIEWWWAR LNDKEGYVPR NLLGLYPRIK PRQRSLA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1127 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.