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Polyclonal Rabbit anti-Human TP53BP2 / ASPP2 Antibody (IHC, WB)

Anti-ASPP2 Antibody - Western Blot. Western blot of Protein A purified anti-ASPP2 to detect over-expressed ASPP2 in MCF-7 cells (Lane 2, arrowhead). Lane 1 is a nontransfected control. Lane 3 is MCF-7 cells over-expressing ASPP1. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1000 dilution. The identity of the lower MW band at approximately 40kD is unknown. Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.

Polyclonal Rabbit anti-Human TP53BP2 / ASPP2 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human TP53BP2 / ASPP2 antibodies →
$579.00each
Catalog Number: LS-C59554-100

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Overview

Description
ASPP2 antibody LS-C59554 is an unconjugated rabbit polyclonal antibody to human ASPP2 (TP53BP2). Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Predicted Reactivity
Cynomolgus Monkey, Dog
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
53BP2 | p53-binding protein 53bp2 | p53BP2 | TP53BP2 | PPP1R13A | ASPP2 | BBP | BCL2-binding protein | p53-binding protein 2
Recommended Dilution: ELISA
1:2500 - 1:10000
Recommended Dilution: WB
1:500 - 1:3000
UniProt Number
Q13625
SwissProt
Q13625

Target

Target
Human TP53BP2 / ASPP2
Antigen Species
Human
Epitope
This Protein A purified antibody is directed against human ASPP2. Minimal reactivity occurs against ASPP1. A BLAST analysis was used to suggest cross-reactivity with ASPP2 from macaque and dog sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with ASPP2 from mouse and rat sources based on an 80% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Gene Name
TP53BP2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP2.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
2.4 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: -20°C; Long term: -20°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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