Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TP53BP2 / ASPP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120459-100
Catalog Number: LS-G120459
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TP53BP2(TP53BP2) (NM_001031685), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 53BP2 | p53-binding protein 53bp2 | p53BP2 | TP53BP2 | PPP1R13A | ASPP2 | BBP | BCL2-binding protein | p53-binding protein 2
- Origin Species
- Human
- NCBI GenBank
- NM_005426
- SwissProt
- Q13625
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 126.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPMFLTVYLS NNEQHFTEVP VTPETICRDV VDLCKEPGES DCHLAEVWCG SERPVADNER61MFDVLQRFGS QRNEVRFFLR HERPPGRDIV SGPRSQDPSL KRNGVKVPGE YRRKENGVNS121PRMDLTLAEL QEMASRQQQQ IEAQQQLLAT KEQRLKFLKQ QDQRQQQQVA EQEKLKRLKE181IAENQEAKLK KVRALKGHVE QKRLSNGKLV EEIEQMNNLF QQKQRELVLA VSKVEELTRQ241LEMLKNGRID SHHDNQSAVA ELDRLYKELQ LRNKLNQEQN AKLQQQRECL NKRNSEVAVM301DKRVNELRDR LWKKKAALQQ KENLPVSSDG NLPQQAASAP SRVAAVGPYI QSSTMPRMPS361RPELLVKPAL PDGSLVIQAS EGPMKIQTLP NMRSGAASQT KGSKIHPVGP DWSPSNADLF421PSQGSASVPQ STGNALDQVD DGEVPLREKE KKVRPFSMFD AVDQSNAPPS FGTLRKNQSS481EDILRDAQVA NKNVAKVPPP VPTKPKQINL PYFGQTNQPP SDIKPDGSSQ QLSTVVPSMG541TKPKPAGQQP RVLLSPSIPS VGQDQTLSPG SKQESPPAAA VRPFTPQPSK DTLLPPFRKP601QTVAASSIYS MYTQQQAPGK NFQQAVQSAL TKTHTRGPHF SSVYGKPVIA AAQNQQQHPE661NIYSNSQGKP GSPEPETEPV SSVQENHENE RIPRPLSPTK LLPFLSNPYR NQSDADLEAL721RKKLSNAPRP LKKRSSITEP EGPNGPNIQK LLYQRTTIAA METISVPSYP SKSASVTASS781ESPVEIQNPY LHVEPEKEVV SLVPESLSPE DVGNASTENS DMPAPSPGLD YEPEGVPDNS841PNLQNNPEEP NPEAPHVLDV YLEEYPPYPP PPYPSGEPEG PGEDSVSMRP PEITGQVSLP901PGKRTNLRKT GSERIAHGMR VKFNPLALLL DSSLEGEFDL VQRIIYEVDD PSLPNDEGIT961ALHNAVCAGH TEIVKFLVQF GVNVNAADSD GWTPLHCAAS CNNVQVCKFL VESGAAVFAM1021TYSDMQTAAD KCEEMEEGYT QCSQFLYGVQ EKMGIMNKGV IYALWDYEPQ NDDELPMKEG1081DCMTIIHRED EDEIEWWWAR LNDKEGYVPR NLLGLYPRIK PRQRSLATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1127 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.