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Human SORCS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SORCS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G86739-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SORCS2 (NM_020777) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1329 | SORCS2 | VPS10 domain receptor protein
Origin Species
Human
NCBI GenBank
AB037750
SwissProt
Q96PQ0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
128 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAHRGPSRAS KGPGPTARAP SPGAPPPPRS PRSRPLLLLL LLLGACGAAG RSPEPGRLGP
61HAQLTRVPRS PPAGRAEPGG GEDRQARGTE PGAPGPSPGP APGPGEDGAP AAGYRRWERA
121APLAGVASRA QVSLISTSFV LKGDATHNQA MVHWTGENSS VILILTKYYH ADMGKVLESS
181LWRSSDFGTS YTKLTLQPGV TTVIDNFYIC PTNKRKVILV SSSLSDRDQS LFLSADEGAT
241FQKQPIPFFV ETLIFHPKEE DKVLAYTKES KLYVSSDLGK KWTLLQERVT KDHVFWSVSG
301VDADPDLVHV EAQDLGGDFR YVTCAIHNCS EKMLTAPFAG PIDHGSLTVQ DDYIFFKATS
361ANQTKYYVSY RRNEFVLMKL PKYALPKDLQ IISTDESQVF VAVQEWYQMD TYNLYQSDPR
421GVRYALVLQD VRSSRQAEES VLIDILEVRG VKGVFLANQK IDGKVMTLIT YNKGRDWDYL
481RPPSMDMNGK PTNCKPPDCH LHLHLRWADN PYVSGTVHTK DTAPGLIMGA GNLGSQLVEY
541KEEMYITSDC GHTWRQVFEE EHHILYLDHG GVIVAIKDTS IPLKILKFSV DEGLTWSTHN
601FTSTSVFVDG LLSEPGDETL VMTVFGHISF RSDWELVKVD FRPSFSRQCG EEDYSSWELS
661NLQGDRCIMG QQRSFRKRKS TSWCIKGRSF TSALTSRVCE CRDSDFLCDY GFERSSSSES
721STNKCSANFW FNPLSPPDDC ALGQTYTSSL GYRKVVSNVC EGGVDMQQSQ VQLQCPLTPP
781RGLQVSIQGE AVAVRPGEDV LFVVRQEQGD VLTTKYQVDL GDGFKAMYVN LTLTGEPIRH
841RYESPGIYRV SVRAENTAGH DEAVLFVQVN SPLQALYLEV VPVIGLNQEV NLTAVLLPLN
901PNLTVFYWWI GHSLQPLLSL DNSVTTRFSD TGDVRVTVQA ACGNSVLQDS RVLRVLDQFQ
961VMPLQFSKEL DAYNPNTPEW REDVGLVVTR LLSKETSVPQ ELLVTVVKPG LPTLADLYVL
1021LPPPRPTRKR SLSSDKRLAA IQQVLNAQKI SFLLRGGVRV LVALRDTGTG AEQLGGGGGY
1081WAVVVLFVIG LFAAGAFILY KFKRKRPGRT VYAQMHNEKE QEMTSPVSHS EDVQGAVQGN
1141HSGVVLSINS REMHSYLVS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1159 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.