Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HSorCS / SORCS1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102154-100
Catalog Number: LS-G102154
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SORCS1 (NM_052918), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HSorCS | SORCS | SORCS1
- Origin Species
- Human
- SwissProt
- Q8WY21
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 129.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGKVGAGGGS QARLSALLAG AGLLILCAPG VCGGGSCCPS PHPSSAPRSA STPRGFSHQG61RPGRAPATPL PLVVRPLFSV APGDRALSLE RARGTGASMA VAARSGRRRR SGADQEKAER121GEGASRSPRG VLRDGGQQEP GTRERDPDKA TRFRMEELRL TSTTFALTGD SAHNQAMVHW181SGHNSSVILI LTKLYDYNLG SITESSLWRS TDYGTTYEKL NDKVGLKTIL SYLYVCPTNK241RKIMLLTDPE IESSLLISSD EGATYQKYRL NFYIQSLLFH PKQEDWILAY SQDQKLYSSA301EFGRRWQLIQ EGVVPNRFYW SVMGSNKEPD LVHLEARTVD GHSHYLTCRM QNCTEANRNQ361PFPGYIDPDS LIVQDHYVFV QLTSGGRPHY YVSYRRNAFA QMKLPKYALP KDMHVISTDE421NQVFAAVQEW NQNDTYNLYI SDTRGVYFTL ALENVQSSRG PEGNIMIDLY EVAGIKGMFL481ANKKIDNQVK TFITYNKGRD WRLLQAPDTD LRGDPVHCLL PYCSLHLHLK VSENPYTSGI541IASKDTAPSI IVASGNIGSE LSDTDISMFV SSDAGNTWRQ IFEEEHSVLY LDQGGVLVAM601KHTSLPIRHL WLSFDEGRSW SKYSFTSIPL FVDGVLGEPG EETLIMTVFG HFSHRSEWQL661VKVDYKSIFD RRCAEEDYRP WQLHSQGEAC IMGAKRIYKK RKSERKCMQG KYAGAMESEP721CVCTEADFDC DYGYERHSNG QCLPAFWFNP SSLSKDCSLG QSYLNSTGYR KVVSNNCTDG781VREQYTAKPQ KCPGKAPRGL RIVTADGKLT AEQGHNVTLM VQLEEGDVQR TLIQVDFGDG841IAVSYVNLSS MEDGIKHVYQ NVGIFRVTVQ VDNSLGSDSA VLYLHVTCPL EHVHLSLPFV901TTKNKEVNAT AVLWPSQVGT LTYVWWYGNN TEPLITLEGS ISFRFTSEGM NTITVQVSAG961NAILQDTKTI AVYEEFRSLR LSFSPNLDDY NPDIPEWRRD IGRVIKKSLV EATGVPGQHI1021LVAVLPGLPT TAELFVLPYQ DPAGENKRST DDLEQISELL IHTLNQNSVH FELKPGVRVL1081VHAAHLTAAP LVDLTPTHSG SAMLMLLSVV FVGLAVFVIY KFKRRVALPS PPSPSTQPGD1141SSLRLQRARH ATPPSTPKRG SAGAQYAITRTRPLEQKLISEEDLAANDILDYKDDDDKV
1168 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.