LSBio
Human METTL13 / KIAA0859 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human METTL13 / KIAA0859 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all METTL13 antibodies →
$153.00each
Catalog Number: LS-G90181-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0859(METTL13) (NM_015935) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Antiapoptotic protein FEAT | CGI-01 | Feat | Methyltransferase like 13 | METTL13 | KIAA0859
Origin Species
Human
NCBI GenBank
NM_015935
SwissProt
Q8N6R0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNLLPKSSRE FGSVDYWEKF FQQRGKKAFE WYGTYLELCG VLHKYIKPRE KVLVIGCGNS
61ELSEQLYDVG YRDIVNIDIS EVVIKQMKEC NATRRPQMSF LKMDMTQMEF PDASFQVVLD
121KGTLDAVLTD EEEKTLQQVD RMLAEVGRVL QVGGRYLCIS LAQAHILKKA VGHFSREGWM
181VRVHQVANSQ DQVLEAEPQF SLPVFAFIMT KFRPVPGSAL QIFELCAQEQ RKPVRLESAE
241RLAEAVQERQ QYAWLCSQLR RKARLGSVSL DLCDGDTGEP RYTLHVVDSP TVKPSRDNHF
301AIFIIPQGRE TEWLFGMDEG RKQLAASAGF RRLITVALHR GQQYESMDHI QAELSARVME
361LAPAGMPTQQ QVPFLSVGGD IGVRTVQHQD CSPLSGDYVI EDVQGDDKRY FRRLIFLSNR
421NVVQSEARLL KDVSHKAQKK RKKDRKKQRP ADAEDLPAAP GQSIDKSYLC CEHHKAMIAG
481LALLRNPELL LEIPLALLVV GLGGGSLPLF VHDHFPKSCI DAVEIDPSML EVATQWFGFS
541QSDRMKVHIA DGLDYIASLA GGGEARPCYD VIMFDVDSKD PTLGMSCPPP AFVEQSFLQK
601VKSILTPEGV FILNLVCRDL GLKDSVLAGL KAVFPLLYVR RIEGEVNEIL FCQLHPEQKL
661ATPELLETAQ ALERTLRKPG RGWDDTYVLS DMLKTVKIV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

699 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.