Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
METTL13 / KIAA0859 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112964-100
Catalog Number: LS-G112964
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0859(METTL13) (NM_015935), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Antiapoptotic protein FEAT | CGI-01 | Feat | Methyltransferase like 13 | METTL13 | KIAA0859
- Origin Species
- Human
- NCBI GenBank
- NM_015935
- SwissProt
- Q8N6R0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNLLPKSSRE FGSVDYWEKF FQQRGKKAFE WYGTYLELCG VLHKYIKPRE KVLVIGCGNS61ELSEQLYDVG YRDIVNIDIS EVVIKQMKEC NATRRPQMSF LKMDMTQMEF PDASFQVVLD121KGTLDAVLTD EEEKTLQQVD RMLAEVGRVL QVGGRYLCIS LAQAHILKKA VGHFSREGWM181VRVHQVANSQ DQVLEAEPQF SLPVFAFIMT KFRPVPGSAL QIFELCAQEQ RKPVRLESAE241RLAEAVQERQ QYAWLCSQLR RKARLGSVSL DLCDGDTGEP RYTLHVVDSP TVKPSRDNHF301AIFIIPQGRE TEWLFGMDEG RKQLAASAGF RRLITVALHR GQQYESMDHI QAELSARVME361LAPAGMPTQQ QVPFLSVGGD IGVRTVQHQD CSPLSGDYVI EDVQGDDKRY FRRLIFLSNR421NVVQSEARLL KDVSHKAQKK RKKDRKKQRP ADAEDLPAAP GQSIDKSYLC CEHHKAMIAG481LALLRNPELL LEIPLALLVV GLGGGSLPLF VHDHFPKSCI DAVEIDPSML EVATQWFGFS541QSDRMKVHIA DGLDYIASLA GGGEARPCYD VIMFDVDSKD PTLGMSCPPP AFVEQSFLQK601VKSILTPEGV FILNLVCRDL GLKDSVLAGL KAVFPLLYVR RIEGEVNEIL FCQLHPEQKL661ATPELLETAQ ALERTLRKPG RGWDDTYVLS DMLKTVKIVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
699 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.