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METTL2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

METTL2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111739-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human METTL2B (NM_018396), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Methyltransferase like 2B | METTL2B | Methyltransferase like 2 | METTL2 | PSENIP1 | METL
Origin Species
Human
NCBI GenBank
NM_018396
SwissProt
Q6P1Q9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
43.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MAGSYPEGAP AVLADKRQQF GSRFLRDPAR VFHHNAWDNV EWSEEQAAAA ERKVQENSIQ
61RVCQEKQVDY EINAHKYWND FYKIHENGFF KDRHWLFTEF PELAPSQNQN HLKDWFLENK
121SEVPECRNNE DGPGLIMEEQ HKCSSKSLEH KTQTPPVEEN VTQKISDLEI CADEFPGSSA
181TYRILEVGCG VGNTVFPILQ TNNDPGLFVY CCDFSSTAIE LVQTNSEYDP SRCFAFVHDL
241CDEEKSYPVP KGSLDIIILI FVLSAIVPDK MQKAINRLSR LLKPGGMMLL RDYGRYDMAQ
301LRFKKGQCLS GNFYVRGDGT RVYFFTQEEL DTLFTTAGLE KVQNLVDRRL QVNRGKQLTM
361YRVWIQCKYC KPLLSSTS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

378 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.