GSPT1 Antibody
Catalog Number: LS-C801739
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human GSPT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G88079-20
Catalog Number: LS-G88079
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GSPT1(GSPT1) (NM_001130006) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 551G9.2 | ETF3A | GSPT1 | ERF3a | G1 to S phase transition 1 | GST1
- Origin Species
- Human
- NCBI GenBank
- NM_002094
- SwissProt
- P15170
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPGSGGGGG GGGGGGSSSG SSSSDSAPDC WDQADMEAPG PGPCGGGGSL AAAAEAQREN61LSAAFSRQLN VNAKPFVPNV HAAEFVPSFL RGPAAPPPPA GGAANNHGAG SGAGGRAAPV121ESSQEEQSLC EGSNSAVSME LSEPIENGET EMSPEESWEH KEEISEAEPG GGSLGDGRPP181EESAHEMMEE EEEIPKPKSV VAPPGAPKKE HVNVVFIGHV DAGKSTIGGQ IMYLTGMVDK241RTLEKYEREA KEKNRETWYL SWALDTNQEE RDKGKTVEVG RAYFETEKKH FTILDAPGHK301SFVPNMIGGA SQADLAVLVI SARKGEFETG FEKGGQTREH AMLAKTAGVK HLIVLINKMD361DPTVNWSNER YEECKEKLVP FLKKVGFNPK KDIHFMPCSG LTGANLKEQS DFCPWYIGLP421FIPYLDNLPN FNRSVDGPIR LPIVDKYKDM GTVVLGKLES GSICKGQQLV MMPNKHNVEV481LGILSDDVET DTVAPGENLK IRLKGIEEEE ILPGFILCDP NNLCHSGRTF DAQIVIIEHK541SIICPGYNAV LHIHTCIEEV EITALICLVD KKSGEKSKTR PRFVKQDQVC IARLRTAGTI601CLETFKDFPQ MGRFTLRDEG KTIAIGKVLK LVPEKDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
636 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
