GSPT1 Antibody
Catalog Number: LS-C801739
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human GSPT1 Protein (Over-Expression Lysate Myc-DDK (Flag), C-terminus)
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129072-100
Catalog Number: LS-G129072
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GSPT1(GSPT1) (NM_002094), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 551G9.2 | ETF3A | GSPT1 | ERF3a | G1 to S phase transition 1 | GST1
- Origin Species
- Human
- NCBI GenBank
- NM_002094
- SwissProt
- P15170
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPGSGGGGG GGGGGGSSSG SSSSDSAPDC WDQADMEAPG PGPCGGGGSL AAAAEAQREN61LSAAFSRQLN VNAKPFVPNV HAAEFVPSFL RGPAAPPPPA GGAANNHGAG SGAGGRAAPV121ESSQEEQSLC EGSNSAVSME LSEPIVENGE TEMSPEESWE HKEEISEAEP GGGSLGDGRP181PEESAHEMME EEEEIPKPKS VVAPPGAPKK EHVNVVFIGH VDAGKSTIGG QIMYLTGMVD241KRTLEKYERE AKEKNRETWY LSWALDTNQE ERDKGKTVEV GRAYFETEKK HFTILDAPGH301KSFVPNMIGG ASQADLAVLV ISARKGEFET GFEKGGQTRE HAMLAKTAGV KHLIVLINKM361DDPTVNWSNE RYEECKEKLV PFLKKVGFNP KKDIHFMPCS GLTGANLKEQ SDFCPWYIGL421PFIPYLDNLP NFNRSVDGPI RLPIVDKYKD MGTVVLGKLE SGSICKGQQL VMMPNKHNVE481VLGILSDDVE TDTVAPGENL KIRLKGIEEE EILPGFILCD PNNLCHSGRT FDAQIVIIEH541KSIICPGYNA VLHIHTCIEE VEITALICLV DKKSGEKSKT RPRFVKQDQV CIARLRTAGT601ICLETFKDFP QMGRFTLRDE GKTIAIGKVL KLVPEKDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
637 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
