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GSPT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GSPT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G124942-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GSPT1(GSPT1) (NM_001130006), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
551G9.2 | ETF3A | GSPT1 | ERF3a | G1 to S phase transition 1 | GST1
Origin Species
Human
NCBI GenBank
NM_002094
SwissProt
P15170

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MDPGSGGGGG GGGGGGSSSG SSSSDSAPDC WDQADMEAPG PGPCGGGGSL AAAAEAQREN
61LSAAFSRQLN VNAKPFVPNV HAAEFVPSFL RGPAAPPPPA GGAANNHGAG SGAGGRAAPV
121ESSQEEQSLC EGSNSAVSME LSEPIENGET EMSPEESWEH KEEISEAEPG GGSLGDGRPP
181EESAHEMMEE EEEIPKPKSV VAPPGAPKKE HVNVVFIGHV DAGKSTIGGQ IMYLTGMVDK
241RTLEKYEREA KEKNRETWYL SWALDTNQEE RDKGKTVEVG RAYFETEKKH FTILDAPGHK
301SFVPNMIGGA SQADLAVLVI SARKGEFETG FEKGGQTREH AMLAKTAGVK HLIVLINKMD
361DPTVNWSNER YEECKEKLVP FLKKVGFNPK KDIHFMPCSG LTGANLKEQS DFCPWYIGLP
421FIPYLDNLPN FNRSVDGPIR LPIVDKYKDM GTVVLGKLES GSICKGQQLV MMPNKHNVEV
481LGILSDDVET DTVAPGENLK IRLKGIEEEE ILPGFILCDP NNLCHSGRTF DAQIVIIEHK
541SIICPGYNAV LHIHTCIEEV EITALICLVD KKSGEKSKTR PRFVKQDQVC IARLRTAGTI
601CLETFKDFPQ MGRFTLRDEG KTIAIGKVLK LVPEKD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

636 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.