Human FEM1A Protein (Recombinant GST)
Catalog Number: LS-G34330
2 sizes / from $489.00
Expression System Tobacco / Plant-Based Expression
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human FEM1A Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G82460-20
Catalog Number: LS-G82460
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FEM1A (NM_018708) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EPRAP | Fem-1 homolog a (C. elegans) | FEM1A | FEM1-alpha | Protein fem-1 homolog A
- Origin Species
- Human
- NCBI GenBank
- NM_018708
- SwissProt
- Q9BSK4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDLRTAVYNA ARDGKLQLLQ KLLSGRSREE LDELTGEVAG GGTPLLIAAR YGHLDVVEYL61VDRCGASVEA GGSVHFDGET IEGAPPLWAA SAAGHLDVVR SLLRRGASVN RTTRTNSTPL121RAACFDGHLE VVRYLVGEHQ ADLEVANRHG HTCLMISCYK GHREIARYLL EQGAQVNRRS181AKGNTALHDC AESGSLEILQ LLLGCKARME RDGYGMTPLL AASVTGHTNI VEYLIQEQPG241QEQVAGGEAQ PGLPQEDPST SQGCAQPQGA PCCSSSPEEP LNGESYESCC PTSREAAVEA301LELLGATYVD KKRDLLGALK HWRRAMELRH QGGEYLPKPE PPQLVLAYDY SREVNTTEEL361EALITDPDEM RMQALLIRER ILGPSHPDTS YYIRYRGAVY ADSGNFERCI RLWKYALDMQ421QSNLEPLSPM TASSFLSFAE LFSYVLQDRA AKGSLGTQIG FADLMGVLTK GVREVERALQ481LPREPGDSAQ FTKALAIILH LLYLLEKVEC TPSQEHLKHQ TVYRLLKCAP RGKNGFTPLH541MAVDKDTTNV GRYPVGRFPS LHVVKVLLDC GADPDSRDFD NNTPLHIAAQ NNCPAIMNAL601IEAGAHMDAT NAFKKTAYEL LDEKLLARGT MQPFNYVTLQ CLAARALDKN KIPYKGFIPE661DLEAFIELHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
669 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
