LSBio
FEM1A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FEM1A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FEM1A antibodies →
$504.00each
Catalog Number: LS-G111580-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FEM1A (NM_018708), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EPRAP | Fem-1 homolog a (C. elegans) | FEM1A | FEM1-alpha | Protein fem-1 homolog A
Origin Species
Human
NCBI GenBank
NM_018708
SwissProt
Q9BSK4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLRTAVYNA ARDGKLQLLQ KLLSGRSREE LDELTGEVAG GGTPLLIAAR YGHLDVVEYL
61VDRCGASVEA GGSVHFDGET IEGAPPLWAA SAAGHLDVVR SLLRRGASVN RTTRTNSTPL
121RAACFDGHLE VVRYLVGEHQ ADLEVANRHG HTCLMISCYK GHREIARYLL EQGAQVNRRS
181AKGNTALHDC AESGSLEILQ LLLGCKARME RDGYGMTPLL AASVTGHTNI VEYLIQEQPG
241QEQVAGGEAQ PGLPQEDPST SQGCAQPQGA PCCSSSPEEP LNGESYESCC PTSREAAVEA
301LELLGATYVD KKRDLLGALK HWRRAMELRH QGGEYLPKPE PPQLVLAYDY SREVNTTEEL
361EALITDPDEM RMQALLIRER ILGPSHPDTS YYIRYRGAVY ADSGNFERCI RLWKYALDMQ
421QSNLEPLSPM TASSFLSFAE LFSYVLQDRA AKGSLGTQIG FADLMGVLTK GVREVERALQ
481LPREPGDSAQ FTKALAIILH LLYLLEKVEC TPSQEHLKHQ TVYRLLKCAP RGKNGFTPLH
541MAVDKDTTNV GRYPVGRFPS LHVVKVLLDC GADPDSRDFD NNTPLHIAAQ NNCPAIMNAL
601IEAGAHMDAT NAFKKTAYEL LDEKLLARGT MQPFNYVTLQ CLAARALDKN KIPYKGFIPE
661DLEAFIELH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

669 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.