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FEM1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FEM1B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FEM1B antibodies →
$504.00each
Catalog Number: LS-G113290-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FEM 1B(FEM1B) (NM_015322), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
F1AA | FEM-1 (C.elegans) homolog b | FEM1-beta | FIAA | KIAA0396 | F1A-ALPHA | Fem-1 homolog b (C. elegans) | FEM1B | Protein fem-1 homolog B
Origin Species
Human
NCBI GenBank
NM_015322
SwissProt
Q9UK73

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGLAGYVYK AASEGKVLTL AALLLNRSES DIRYLLGYVS QQGGQRSTPL IIAARNGHAK
61VVRLLLEHYR VQTQQTGTVR FDGYVIDGAT ALWCAAGAGH FEVVKLLVSH GANVNHTTVT
121NSTPLRAACF DGRLDIVKYL VENNANISIA NKYDNTCLMI AAYKGHTDVV RYLLEQRADP
181NAKAHCGATA LHFAAEAGHI DIVKELIKWR AAIVVNGHGM TPLKVAAESC KADVVELLLS
241HADCDRRSRI EALELLGASF ANDRENYDII KTYHYLYLAM LERFQDGDNI LEKEVLPPIH
301AYGNRTECRN PQELESIRQD RDALHMEGLI VRERILGADN IDVSHPIIYR GAVYADNMEF
361EQCIKLWLHA LHLRQKGNRN THKDLLRFAQ VFSQMIHLNE TVKAPDIECV LRCSVLEIEQ
421SMNRVKNISD ADVHNAMDNY ECNLYTFLYL VCISTKTQCS EEDQCKINKQ IYNLIHLDPR
481TREGFTLLHL AVNSNTPVDD FHTNDVCSFP NALVTKLLLD CGAEVNAVDN EGNSALHIIV
541QYNRPISDFL TLHSIIISLV EAGAHTDMTN KQNKTPLDKS TTGVSEILLK TQMKMSLKCL
601AARAVRANDI NYQDQIPRTL EEFVGFH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

627 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.