LSBio
Human FAM198B / C4orf18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FAM198B / C4orf18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FAM198B antibodies →
$153.00each
Catalog Number: LS-G90497-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FAM198B (NM_001031700) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AD021 | AD036 | ENED | C4orf18 | FAM198B | Protein FAM198B
Origin Species
Human
NCBI GenBank
NM_016613
SwissProt
Q6UWH4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTCPDKPGQL INWFICSLCV PRVRKLWSSR RPRTRRNLLL GTACAIYLGF LVSQVGRASL
61QHGQAAEKGP HRSRDTAEPS FPEIPLDGTL APPESQGNGS TLQPNVVYIT LRSKRSKPAN
121IRGTVKPKRR KKHAVASAAP GQEALVGPSL QPQEAAREAD AVAPGYAQGA NLVKIGERPW
181RLVRGPGVRA GGPDFLQPSS RESNIRIYSE SAPSWLSKDD IRRMRLLADS AVAGLRPVSS
241RSGARLLVLE GGAPGAVLRC GPSPCGLLKQ PLDMSEVFAF HLDRILGLNR TLPSVSRKAE
301FIQAAAAACL SMRLAFFIFP AWEDMRVPGK CCIGHARLTW GTYQQLLKQK CWQNGRVPKP
361ESGCTEIHHH EWSKMALFDF LLQIYNRLDT NCCGFRPRKE DACVQNGLRP KCDDQGSAAL
421AHIIQRKHDP RHLVFIDNKS FFDRSEDNLN FKLLEGIKEF PASAVSVLKS QHLRQKLLQS
481LFLDKVYWES QGGRQGIEKL IDVIEHRAKI LITYINAHGV KVLPMNE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

527 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.