Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM198B / C4orf18 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120701-100
Catalog Number: LS-G120701
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM198B (NM_001031700), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AD021 | AD036 | ENED | C4orf18 | FAM198B | Protein FAM198B
- Origin Species
- Human
- NCBI GenBank
- NM_016613
- SwissProt
- Q6UWH4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTCPDKPGQL INWFICSLCV PRVRKLWSSR RPRTRRNLLL GTACAIYLGF LVSQVGRASL61QHGQAAEKGP HRSRDTAEPS FPEIPLDGTL APPESQGNGS TLQPNVVYIT LRSKRSKPAN121IRGTVKPKRR KKHAVASAAP GQEALVGPSL QPQEAAREAD AVAPGYAQGA NLVKIGERPW181RLVRGPGVRA GGPDFLQPSS RESNIRIYSE SAPSWLSKDD IRRMRLLADS AVAGLRPVSS241RSGARLLVLE GGAPGAVLRC GPSPCGLLKQ PLDMSEVFAF HLDRILGLNR TLPSVSRKAE301FIQAAAAACL SMRLAFFIFP AWEDMRVPGK CCIGHARLTW GTYQQLLKQK CWQNGRVPKP361ESGCTEIHHH EWSKMALFDF LLQIYNRLDT NCCGFRPRKE DACVQNGLRP KCDDQGSAAL421AHIIQRKHDP RHLVFIDNKS FFDRSEDNLN FKLLEGIKEF PASAVSVLKS QHLRQKLLQS481LFLDKVYWES QGGRQGIEKL IDVIEHRAKI LITYINAHGV KVLPMNETRTRPLEQKLISEEDLAANDILDYKDDDDKV
527 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.