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FAM178B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM178B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM178B antibodies →
$504.00each
Catalog Number: LS-G124409-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM178B (NM_001122646), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LOC51252 | Protein FAM178B | FAM178B
Origin Species
Human
NCBI GenBank
NM_016490
SwissProt
Q8IXR5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.85 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGPQETVLA LPLREGVQAA ATVPILLYNL EDGLSDHPLD QGPRCPARRP CSPASAPAPT
61SPKKPKIQAP GETFPTDWSP PPVEFLNPRV LQASREAPAQ RWVGVVGPQG LRRLAGELPE
121ELEQEHLDLD PKRGLALPEK LFWNTSGLSQ QAAAPEFSWG GSGSYFNNLD YLLQEKREQA
181LEQERERLLL QECLNLNSLD LDEEEVPLTP EHRMLVEKYS VSLQTIPPVH PGETVFLPRC
241HPLPCILDSS LLKPRSHLEG LFLSSPPAQQ LSFLRSGLLN ILYLHMPDCP VSLLQWLFQL
301LTWPPETSLG AFGLLWDLIV DGIFLQPDED KHLWCPSLQE VREAFHSLGA HSPALYPLGP
361FWHGGRVLPG EAGLNENEEQ DAPQEIALDI SLGHIYKFLA LCAQAQPGAY TDENLMGLIE
421LLCRTSLDVG LRLLPKVDLQ QLLLLLLENI REWPGKLQEL CCTLSWVSDH HHNLLALVQF
481FPDMTSRSRR LRSQLSLVVI ARMLGQQEML PLWQEKTQLS SLSRLLGLMR PSSLRQYLDS
541VPLPPCQEQQ PKASAELDHK ACYLCHSLLM LAGVVVSCQD ITPDQWGELQ LLCMQLDRHI
601STQIRESPQA MHRTMLKDLA TQTYIRWQEL LTHCQPQAQY FSPWKDI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

647 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.