E4 / UBE4A Antibody
Catalog Number: LS-C661397-100
100 µg / $348.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human E4 / UBE4A Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G76664-20
Catalog Number: LS-G76664
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human UBE4A (NM_004788) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ubiquitination factor E4A | UFD2 | UBOX2 | E4 | KIAA0126 | UBE4A
- Origin Species
- Human
- NCBI GenBank
- NM_004788
- SwissProt
- Q14139
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 123.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS DNSVSESLDE61FDYSVAEISR SFRSQQEICE QLNINHMIQR IFLITLDNSD PSLKSGNGIP SRCVYLEEMA121VELEDQDWLD MSNVEQALFA RLLLQDPGNH LINMTSSTTL NLSADRDAGE RHIFCYLYSC181FQRAKEEITK VPENLLPFAV QCRNLTVSNT RTVLLTPEIY VDQNIHEQLV DLMLEAIQGA241REYMNKIYFE DVTEFLEEVI EALILDEEVR TFPEVMIPVF DILLGRIKDL ELCQILLYAY301LDILLYFTRQ KDMAKVFVEY IQPKDPTNGQ MYQKTLLGVI LSISCLLKTP GVVENHGYFL361NPSRSSPQEI KVQEANIHQF MAQFHEKIYQ MLKNLLQLSP ETKHCILSWL GNCLHANAGR421TKIWANQMPE IFFQMYASDA FFLNLGAALL KLCQPFCKPR SSRLLTFNPT YCALKELNDE481ERKIKNVHMR GLDKETCLIP AVQEPKFPQN YNLVTENLAL TEYTLYLGFH RLHDQMVKIN541QNLHRLQVAW RDAQQSSSPA ADNLREQFER LMTIYLSTKT AMTEPQMLQN CLNLQVSMAV601LLVQLAIGNE GSQPIELTFP LPDGYSSLAY VPEFFADNLG DFLIFLRRFA DDILETSADS661LEHVLHFITI FTGSIERMKN PHLRAKLAEV LEAVMPHLDQ TPNPLVSSVF HRKRVFCNFQ721YAPQLAEALI KVFVDIEFTG DPHQFEQKFN YRRPMYPILR YMWGTDTYRE SIKDLADYAS781KNLEAMNPPL FLRFLNLLMN DAIFLLDEAI QYLSKIKIQQ IEKDRGEWDS LTPEARREKE841AGLQMFGQLA RFHNIMSNET IGTLAFLTSE IKSLFVHPFL AERIISMLNY FLQHLVGPKM901GALKVKDFSE FDFKPQQLVS DICTIYLNLG DEENFCATVP KDGRSYSPTL FAQTVRVLKK961INKPGNMIMA FSNLAERIKS LADLQQQEEE TYADACDEFL DPIMSTLMCD PVVLPSSRVT1021VDRSTIARHL LSDQTDPFNR SPLTMDQIRP NTELKEKIQR WLAERKQQKE QLETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1073 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
