Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
E4 / UBE4A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116412-100
Catalog Number: LS-G116412
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBE4A (NM_004788), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ubiquitination factor E4A | UFD2 | UBOX2 | E4 | KIAA0126 | UBE4A
- Origin Species
- Human
- NCBI GenBank
- NM_004788
- SwissProt
- Q14139
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 123.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS DNSVSESLDE61FDYSVAEISR SFRSQQEICE QLNINHMIQR IFLITLDNSD PSLKSGNGIP SRCVYLEEMA121VELEDQDWLD MSNVEQALFA RLLLQDPGNH LINMTSSTTL NLSADRDAGE RHIFCYLYSC181FQRAKEEITK VPENLLPFAV QCRNLTVSNT RTVLLTPEIY VDQNIHEQLV DLMLEAIQGA241REYMNKIYFE DVTEFLEEVI EALILDEEVR TFPEVMIPVF DILLGRIKDL ELCQILLYAY301LDILLYFTRQ KDMAKVFVEY IQPKDPTNGQ MYQKTLLGVI LSISCLLKTP GVVENHGYFL361NPSRSSPQEI KVQEANIHQF MAQFHEKIYQ MLKNLLQLSP ETKHCILSWL GNCLHANAGR421TKIWANQMPE IFFQMYASDA FFLNLGAALL KLCQPFCKPR SSRLLTFNPT YCALKELNDE481ERKIKNVHMR GLDKETCLIP AVQEPKFPQN YNLVTENLAL TEYTLYLGFH RLHDQMVKIN541QNLHRLQVAW RDAQQSSSPA ADNLREQFER LMTIYLSTKT AMTEPQMLQN CLNLQVSMAV601LLVQLAIGNE GSQPIELTFP LPDGYSSLAY VPEFFADNLG DFLIFLRRFA DDILETSADS661LEHVLHFITI FTGSIERMKN PHLRAKLAEV LEAVMPHLDQ TPNPLVSSVF HRKRVFCNFQ721YAPQLAEALI KVFVDIEFTG DPHQFEQKFN YRRPMYPILR YMWGTDTYRE SIKDLADYAS781KNLEAMNPPL FLRFLNLLMN DAIFLLDEAI QYLSKIKIQQ IEKDRGEWDS LTPEARREKE841AGLQMFGQLA RFHNIMSNET IGTLAFLTSE IKSLFVHPFL AERIISMLNY FLQHLVGPKM901GALKVKDFSE FDFKPQQLVS DICTIYLNLG DEENFCATVP KDGRSYSPTL FAQTVRVLKK961INKPGNMIMA FSNLAERIKS LADLQQQEEE TYADACDEFL DPIMSTLMCD PVVLPSSRVT1021VDRSTIARHL LSDQTDPFNR SPLTMDQIRP NTELKEKIQR WLAERKQQKE QLETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1073 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.